RB-14-0009-500

antibody from RayBiotech, Inc.

Targeting: SET 2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA

ELISA (Recommended by provider)

Antibody Data

Product number

RB-14-0009-500

Provider

RayBiotech, Inc.

Product name

Rabbit anti-SET nuclear oncogene

Antibody type

Polyclonal

Antigen

The imunogen was synthetic peptide.

Description

SET belongs to a family of multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. There are two named isoforms produced by alternative splicing: Isoform 1 and Isoform 2. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography.

Reactivity

Human

Host

Rabbit

Conjugate

Biotin

Antigen sequence

This antibody was produced from a r
abbit immunized with the immunogen.
The IgG fraction was purified from
rabbit serum followed by Protein A
/G affinity chromatography.


Isotype

IgG

Vial size

500 µg

Handling

Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile 1x PBS to a final concentration of 1 mg/ml.