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LearnPA5-78163
antibody from Invitrogen Antibodies
Targeting: SET
2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-78163 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SET Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: A431, H1299, HeLa, HepG2, Molt-4, Raji, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2, C2C12 Predicted Reactivity: Xenopus laevis (99%), Pig (100%), Chicken (100%), Bovine (99%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.43 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SET Polyclonal Antibody detects SET protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SET protein stained by SET Polyclonal Antibody (Product # PA5-78163) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SET Polyclonal Antibody immunoprecipitates SET protein in IP experiments. IP samples: HeLa whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of SET protein by 4 µg SET Polyclonal Antibody (Product # PA5-78163). 10 % SDS-PAGE. The immunoprecipitated SET protein was detected by SET Polyclonal Antibody (Product # PA5-78163) diluted at 1:500.
