MA5-35772

antibody from Invitrogen Antibodies

Targeting: SET 2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-35772

Provider

Invitrogen Antibodies

Product name

SET Recombinant Rabbit Monoclonal Antibody (8E8M9)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ESFFTWFTDH SDAGADELGE VIKDDIWPNP LQYYLVPDMD DEEGEGEEDD DDDEEEEGLE DIDEEGDEDE GEEDEDDDEG EEGEEDEGED D

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

8E8M9

Vial size

100 µL

Concentration

0.2 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of SET in U-2 OS cells. Samples were incubated with SET Monoclonal antibody (Product # MA5-35772) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of SET in 300 μg extracts of 293T cells. Samples were precipitated with 3 μg SET Monoclonal antibody (Product # MA5-35772). Western blot was performed from the immunoprecipitate using SET Monoclonal antibody (Product # MA5-35772) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SET in paraffin-embedded rat brain. Samples were incubated with SET Monoclonal antibody (Product # MA5-35772) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SET in paraffin-embedded human esophageal cancer. Samples were incubated with SET Monoclonal antibody (Product # MA5-35772) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SET in paraffin-embedded mouse spleen. Samples were incubated with SET Monoclonal antibody (Product # MA5-35772) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.