PA5-78023

antibody from Invitrogen Antibodies

Targeting: IL16 FLJ16806, FLJ42735, HsT19289, IL-16, LCF, prIL-16

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78023

Provider

Invitrogen Antibodies

Product name

IL-16 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Control: A549, Jurkat, NT2D1 Predicted Reactivity: Chimpanzee (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.73 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Hsa_circ_0001326 regulates proliferation, migration, invasion, and EMT of HTR-8/SVneo cells via increasing IL16 expression.

Liu Y, Ma X, Liu Y
American journal of reproductive immunology (New York, N.Y. : 1989) 2021 Nov;86(5):e13484

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using IL-16 Polyclonal Antibody (Product # PA5-78023). Sample (30 µg of whole cell lysate). Lane A: A549 . 5% SDS PAGE. IL16 antibody . IL-16 Polyclonal Antibody (Product # PA5-78023) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of IL16 in A549 cells. Samples were incubated with IL16 polyclonal antibody (Product # PA5-78023) using a dilution of 1:500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IL-16 Polyclonal Antibody detects IL16 protein at secreted on human normal colon by immunohistochemical analysis. Sample: Paraffin-embedded human normal colon. IL-16 Polyclonal Antibody (Product # PA5-78023) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

6 FIGURE Identification of IL16 as a target of miR-558. (A) Schematic diagram of the miR-558 binding sites on the IL16 3'UTR. (B) Dual-luciferase reporter assay demonstrated the binding sites between miR-558 and IL16 3'UTR in HTR-8/SVneo cells. (C and D) Analysis of IL16 at mRNA and protein levels in placental tissues of PE patients and healthy controls by RT-qPCR and Western blotting. (E and F) The correlation between IL16 mRNA and miR-558 or circ_0001326 in placental tissues of PE patients was assessed using Pearson's correlation analysis. (G) RT-qPCR analysis of the knockdown efficiency of anti-miR-558 in HTR-8/SVneo cells. (H) Western blotting analysis of the effects of miR-558 overexpression and downregulation on IL16 protein level in HTR-8/SVneo cells. (I) Western blotting analysis of the influence of miR-558 elevation on protein level of IL16 in circ_0001326-overexpressing HTR-8/SVneo cells. ** p < 0.01 and *** p < 0.001