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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-20694 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-20694, RRID:AB_2096342
- Product name
- Anti-ELAVL1
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Respiratory syncytial virus induces host RNA stress granules to facilitate viral replication.
Lindquist ME, Lifland AW, Utley TJ, Santangelo PJ, Crowe JE Jr
Journal of virology 2010 Dec;84(23):12274-84
Journal of virology 2010 Dec;84(23):12274-84
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Supportive validation
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- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 36
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human parathyroid gland shows strong nuclear positivity in glandular cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
