Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00001994-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001994-M02, RRID:AB_538529
- Product name
- Mouse Monoclonal HuR/ELAVL1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ELAVL1 - ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Quantitative proteomic profiling identifies protein correlates to EGFR kinase inhibition.
Kani K, Faca VM, Hughes LD, Zhang W, Fang Q, Shahbaba B, Luethy R, Erde J, Schmidt J, Pitteri SJ, Zhang Q, Katz JE, Gross ME, Plevritis SK, McIntosh MW, Jain A, Hanash S, Agus DB, Mallick P
Molecular cancer therapeutics 2012 May;11(5):1071-81
Molecular cancer therapeutics 2012 May;11(5):1071-81
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HuR/ELAVL1 Antibody (4G8) [H00001994-M02] - ELAVL1 monoclonal antibody (M02), clone 4G8 Analysis of ELAVL1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: HuR/ELAVL1 Antibody (4G8) [H00001994-M02] - Detection limit for recombinant GST tagged ELAVL1 is 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: HuR/ELAVL1 Antibody (4G8) [H00001994-M02] - Analysis of monoclonal antibody to ELAVL1 on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody (4G8) [H00001994-M02] - Analysis of monoclonal antibody to ELAVL1 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.