11910-1-AP
antibody from Invitrogen Antibodies
Targeting: ELAVL1
Hua, HUR, MelG
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [9]
- Immunocytochemistry [2]
- Immunohistochemistry [8]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 11910-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HuR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSNGYEDHM AEDCRGDIGR TNLIVNYLPQ NMTQDELRSL FSSIGEVESA KLIRDKVAGH SLGYGFVNYV TAKDAERAIN TLNGLRLQSK TIKVSYARPS SEVIKDANLY ISGLPRTMTQ KDVEDMFSRF GRIINSRVLV DQTTGLSRGV AFIRFDKRSE AEEAITSFNG HKPPGSSEPI TVKFAANPNQ NKNVALLSQL YHSPARRFGG PVHHQAQRFR FSPMGVDHMS GLSGVNVPGN ASSGWCIFIY NLGQDADEGI LWQMFGPFGA VTNVKVIRDF NTNKCKGFGF VTMTNYEEAA MAIASLNGYR LGDKILQVSF KTNKSHK (1-326 aa encoded by BC003376)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.43 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11910-1-AP (HuR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 11910-1-AP (HuR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11910-1-AP (HuR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11910-1-AP (HuR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HESC cells were subjected to SDS PAGE followed by western blot with 11910-1-AP (HuR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11910-1-AP (HuR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 11910-1-AP (HuR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 11910-1-AP (HuR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11910-1-AP (HuR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using ELAVL1 antibody 11910-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 11910-1-AP (HuR antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor slide using 11910-1-AP (HuR Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas slide using 11910-1-AP (HuR Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas slide using 11910-1-AP (HuR Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer slide using 11910-1-AP (HuR Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer slide using 11910-1-AP (HuR Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin slide using 11910-1-AP (HuR Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin slide using 11910-1-AP (HuR Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor slide using 11910-1-AP ( HuR Antibody) at dilution of 1:50.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 MCF-7 cells were stained with 0.2ug HuR antibody (11910-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-HuR (IP:11910-1-AP, 4ug; Detection:11910-1-AP 1:1000) with HEK-293 cells lysate 2800ug.