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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-7200 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-7200, RRID:AB_649615
- Product name
- Anti-IRS1
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Insulin augments gonadotropin-releasing hormone induction of translation in LbetaT2 cells.
Inactivation of NF-kappaB p50 leads to insulin sensitization in liver through post-translational inhibition of p70S6K.
S6K directly phosphorylates IRS-1 on Ser-270 to promote insulin resistance in response to TNF-(alpha) signaling through IKK2.
Navratil AM, Song H, Hernandez JB, Cherrington BD, Santos SJ, Low JM, Do MH, Lawson MA
Molecular and cellular endocrinology 2009 Nov 13;311(1-2):47-54
Molecular and cellular endocrinology 2009 Nov 13;311(1-2):47-54
Inactivation of NF-kappaB p50 leads to insulin sensitization in liver through post-translational inhibition of p70S6K.
Gao Z, Yin J, Zhang J, He Q, McGuinness OP, Ye J
The Journal of biological chemistry 2009 Jul 3;284(27):18368-76
The Journal of biological chemistry 2009 Jul 3;284(27):18368-76
S6K directly phosphorylates IRS-1 on Ser-270 to promote insulin resistance in response to TNF-(alpha) signaling through IKK2.
Zhang J, Gao Z, Yin J, Quon MJ, Ye J
The Journal of biological chemistry 2008 Dec 19;283(51):35375-82
The Journal of biological chemistry 2008 Dec 19;283(51):35375-82
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 132
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells, cells in molecular layer and cells in granular layer.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
