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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 700662 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRS1 Recombinant Rabbit Monoclonal Antibody (21H10L24)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
- Antibody clone number
- 21H10L24
- Concentration
- 0.5 mg/mL
Submitted references Sustained elevation of MG53 in the bloodstream increases tissue regenerative capacity without compromising metabolic function.
Bian Z, Wang Q, Zhou X, Tan T, Park KH, Kramer HF, McDougal A, Laping NJ, Kumar S, Adesanya TMA, Sermersheim M, Yi F, Wang X, Wu J, Gumpper K, Jiang Q, He D, Lin PH, Li H, Guan F, Zhou J, Kohr MJ, Zeng C, Zhu H, Ma J
Nature communications 2019 Oct 11;10(1):4659
Nature communications 2019 Oct 11;10(1):4659
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IRS-1 in MCF-7 cell lysate (60 µg/lane) using an IRS-1 recombinant rabbit monoclonal antibody (Product # 700662) at a dilution of 5 µg/mL. NBT/BCIP was used as the substrate (Product # WB7105).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IRS-1 in MCF-7 cell lysate (60 µg/lane) using an IRS-1 recombinant rabbit monoclonal antibody (Product # 700662) at a dilution of 5 µg/mL. NBT/BCIP was used as the substrate (Product # WB7105).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Insulin receptor substrate 1 was performed using 70% confluent log phase MIA PaCa-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with IRS1 Recombinant Rabbit Monoclonal Antibody (21H10L24) (Product # 700662) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained withRhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nucleus and cytoplasm localization. Panel e represents Panc-1 (negative model). Panel f represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IRS1 showing staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a IRS1 Recombinant Rabbit Monoclonal Antibody (Product # 700662) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IRS1 showing staining in the cytoplasm of paraffin-embedded human pancreas tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a IRS1 Recombinant Rabbit Monoclonal Antibody (Product # 700662) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
