- 44-813G - Invitrogen Antibodies IRS1 antibody

Submitted references Metformin protects against insulin resistance induced by high uric acid in cardiomyocytes via AMPK signalling pathways in vitro and in vivo.

Jiao Z, Chen Y, Xie Y, Li Y, Li Z
Journal of cellular and molecular medicine 2021 Jul;25(14):6733-6745

Palmitate Is Increased in the Cerebrospinal Fluid of Humans with Obesity and Induces Memory Impairment in Mice via Pro-inflammatory TNF-α.

Melo HM, Seixas da Silva GDS, Sant'Ana MR, Teixeira CVL, Clarke JR, Miya Coreixas VS, de Melo BC, Fortuna JTS, Forny-Germano L, Ledo JH, Oliveira MS, Figueiredo CP, Pardossi-Piquard R, Checler F, Delgado-García JM, Gruart A, Velloso LA, Balthazar MLF, Cintra DE, Ferreira ST, De Felice FG
Cell reports 2020 Feb 18;30(7):2180-2194.e8

Chenodeoxycholic Acid Ameliorates AlCl(3)-Induced Alzheimer's Disease Neurotoxicity and Cognitive Deterioration via Enhanced Insulin Signaling in Rats.

Bazzari FH, Abdallah DM, El-Abhar HS
Molecules (Basel, Switzerland) 2019 May 24;24(10)

Receptor interacting protein 3 protects mice from high-fat diet-induced liver injury.

Roychowdhury S, McCullough RL, Sanz-Garcia C, Saikia P, Alkhouri N, Matloob A, Pollard KA, McMullen MR, Croniger CM, Nagy LE
Hepatology (Baltimore, Md.) 2016 Nov;64(5):1518-1533

Reduction of JNK1 expression with antisense oligonucleotide improves adiposity in obese mice.

Yu XX, Murray SF, Watts L, Booten SL, Tokorcheck J, Monia BP, Bhanot S
American journal of physiology. Endocrinology and metabolism 2008 Aug;295(2):E436-45

Serine phosphorylation proximal to its phosphotyrosine binding domain inhibits insulin receptor substrate 1 function and promotes insulin resistance.

Liu YF, Herschkovitz A, Boura-Halfon S, Ronen D, Paz K, Leroith D, Zick Y
Molecular and cellular biology 2004 Nov;24(21):9668-81

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Extracts prepared from untreated 3T3L-1 (1), insulin-stimulated 3T3-L1 (2), or insulin-stimulated HEK293 cells transfected with wild-type human IRS-1 (lane 3-7) were resolved by SDS-PAGE on an 8% polyacrylamide gel and transferred to PVDF.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Extracts prepared from untreated 3T3L-1 (1), insulin-stimulated 3T3-L1 (2), or insulin-stimulated HEK293 cells transfected with wild-type human IRS-1 (lane 3-7) were resolved by SDS-PAGE on an 8% polyacrylamide gel and transferred to PVDF.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Extracts prepared from untreated 3T3L-1 (1), insulin-stimulated 3T3-L1 (2), or insulin-stimulated HEK293 cells transfected with wild-type human IRS-1 (lane 3-7) were resolved by SDS-PAGE on an 8% polyacrylamide gel and transferred to PVDF.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 7 ( A-B) Glucose tolerance test (GTT) (A) and insulin tolerance test (ITT) (B) in an acute hyperuricaemic mice model. * P < .05 vs. Con and HU+Met. (C-G) Western blot analysis of phospho-AMPK, phospho-IRS1, phospho-Akt and GLUT level in cardiac tissues. (D) * P < .05 vs. Ins+HU. (E) * P < .05 vs. Ins and Ins+HU+Met. (F) * P < .05 vs. Ins and Ins+HU+Met. (G) * P < .05 vs. Ins and Ins+HU+Met. Data are mean +- SD from 4 separate experiments. (H) Schematic representation of how metformin ameliorates HUA-induced insulin resistance in cardiomyocytes. After treatment with HUA, which activates phosphorylation of IRS1 (Ser307). This activity impairs AKT (Ser473) phosphorylation, for insulin resistance. After treatment with metformin, the phosphorylation of AMPK is increased. At the same time, this activity reverses the inhibition of HUA-induced AKT phosphorylation, which ameliorates HUA-induced insulin resistance. HU: hyperuricaemia. Met: metformin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 7 FIGURE ( A-B) Glucose tolerance test (GTT) (A) and insulin tolerance test (ITT) (B) in an acute hyperuricaemic mice model. * P < .05 vs. Con and HU+Met. (C-G) Western blot analysis of phospho-AMPK, phospho-IRS1, phospho-Akt and GLUT level in cardiac tissues. (D) * P < .05 vs. Ins+HU. (E) * P < .05 vs. Ins and Ins+HU+Met. (F) * P < .05 vs. Ins and Ins+HU+Met. (G) * P < .05 vs. Ins and Ins+HU+Met. Data are mean +- SD from 4 separate experiments. (H) Schematic representation of how metformin ameliorates HUA-induced insulin resistance in cardiomyocytes. After treatment with HUA, which activates phosphorylation of IRS1 (Ser307). This activity impairs AKT (Ser473) phosphorylation, for insulin resistance. After treatment with metformin, the phosphorylation of AMPK is increased. At the same time, this activity reverses the inhibition of HUA-induced AKT phosphorylation, which ameliorates HUA-induced insulin resistance. HU: hyperuricaemia. Met: metformin

44-813G

Antibody data