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Western blot
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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
- Other assay [6]
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- Product number
- PA1-1058 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-1058 detects a IRS-1 in mouse and human cells. PA1-1058 has been successfully used in Western blot procedures. By Western blot this antibody detects an ~170 kDa protein representing IRS-1 in 3T3 L1 cell extract. The PA1-1058 immunogen is a synthetic peptide corresponding to residues L(602) H T D D G Y M P M S P G V(615) of human IRS-1. The immunizing peptide is 92% conserved in rats, and mice. This peptide (Cat. # PEP-192) is available for use in neutralization and control experiments.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The role of Smad4 in the regulation of insulin resistance, inflammation and cell proliferation in HTR8-Svneo cells.
Interaction between serine phosphorylated IRS-1 and beta1-integrin affects the stability of neuronal processes.
The hyperglycemia-induced inflammatory response in adipocytes: the role of reactive oxygen species.
Li L, Bai Y, Du R, Tang L, Li L
Cell biochemistry and function 2021 Jan;39(1):126-138
Cell biochemistry and function 2021 Jan;39(1):126-138
Interaction between serine phosphorylated IRS-1 and beta1-integrin affects the stability of neuronal processes.
Wang JY, Gualco E, Peruzzi F, Sawaya BE, Passiatore G, Marcinkiewicz C, Staniszewska I, Ferrante P, Amini S, Khalili K, Reiss K
Journal of neuroscience research 2007 Aug 15;85(11):2360-73
Journal of neuroscience research 2007 Aug 15;85(11):2360-73
The hyperglycemia-induced inflammatory response in adipocytes: the role of reactive oxygen species.
Lin Y, Berg AH, Iyengar P, Lam TK, Giacca A, Combs TP, Rajala MW, Du X, Rollman B, Li W, Hawkins M, Barzilai N, Rhodes CJ, Fantus IG, Brownlee M, Scherer PE
The Journal of biological chemistry 2005 Feb 11;280(6):4617-26
The Journal of biological chemistry 2005 Feb 11;280(6):4617-26
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of IRS1 was done on MCF7 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with IRS1 Rabbit Polyclonal Antibody (PA11058, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 2 FIGURE The deficiency of Smad4 elevated the insulin sensitivity in a cellular model of human insulin resistance. A, The validation of down-regulated Smad4 by western-blot. B, The glucose consumption was determined by commercial kit. C-G, The expression levels of p-IRbeta Tyr1361 , IRbeta, p-IRS-1 Ser307 , p-IRS-1 Tyr612 , IRS-1,Akt and p-Akt Ser473 were determined by western-blot along with relative quantification. H and I, The expressions of GLUT1 and GLUT4 were determined by western-blot along with relative quantification. Values are expressed as mean +- SD. n = 3. * P < .05, ** P < .01, *** P < .001, **** P < .0001
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 3 FIGURE Over-expression of Smad4 reduced the insulin sensitivity in a cellular model of human insulin resistance. A, The validation of over-expressed Smad4 by western-blot along with relative quantification. B, The glucose consumption was determined by commercial kit. C-G, The expression levels of p-IRbeta Tyr1361 , IRbeta, p-IRS-1 Ser307 , p-IRS-1 Tyr612 , IRS-1, Akt and p-Akt Ser473 were determined by western-blot along with relative quantification. H and I, The expressions of GLUT1 and GLUT4 were determined by western-blot along with relative quantification. Values are expressed as mean +- SD. n = 3. * P < .05, ** P < .01, *** P < .001, **** P < .0001
