PA5-36719

antibody from Invitrogen Antibodies

Targeting: IRS1 HIRS-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36719

Provider

Invitrogen Antibodies

Product name

Phospho-IRS1 (Ser1101) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 132 and 180 kDa.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-IRS1 (Ser1101) in Lane 1: A375 whole cell lysate (40 µg), Lane 2: Panc1 whole cell lysate (40 µg), Lane 3: MCF-7 whole cell lysate (40 µg). Samples were incubated with Phospho-IRS1 (Ser1101) polyclonal antibody (Product # PA5-36719) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-IRS1 pSer1101 using Phospho-IRS1 pSer1101 polyclonal antibody (Product # PA5-36719) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with insulin (100nM, 15min), Lane 2: Raw264.7 cell lysate treated with insulin (100nM, 15min), Lane 3: PC12 cell lysate treated with insulin (100nM, 15min).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-IRS1 pSer1101 in paraffin-embedded human colorectal carcinoma using Phospho-IRS1 pSer1101 polyclonal antibody (Product # PA5-36719) at a dilution of 1:50.