Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-36739 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-IRS1 (Ser323) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 132 and 180 kDa.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-IRS1 (Ser323) in Lane 1: HeLa cell lysate treated with insulin, Lane 2: HepG2 cell lysate treated with insulin, Lane 3: Raw264.7 cell lysate treated with insulin. Samples were incubated with Phospho-IRS1 (Ser323) polyclonal antibody (Product # PA5-36739) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-IRS1 pSer323 using Phospho-IRS1 pSer323 polyclonal antibody (Product # PA5-36739) at a dilution of 1:500. Lane 1: Hela cell lysate treated with insulin, Lane 2: HepG2 cell lysate treated with insulin, Lane 3: Raw264.7 cell lysate treated with insulin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-IRS1 (Ser323) in Lane 1: L02 whole cell lysate (40 µg), Lane 2: L02 treated with PBS (1×PBS,PH7.4) for 1 hour whole cell lysate, Lane 3: L02 treated with PBS (1×PBS,PH7.4) for 1 hour then treated with DMEM (10%FBS) for 5min whole cell lysate, Lane 4: L02 treated with PBS (1×PBS,PH7.4) for 1 hour then treated with DMEM (10%FBS) for 10min whole cell lysate, Lane 5: L02 treated with PBS (1×PBS,PH7.4) for 1 hour then treated with DMEM (10%FBS) for 15min whole cell lysate. Samples were incubated with Phospho-IRS1 (Ser323) polyclonal antibody (Product # PA5-36739) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-IRS1 (Ser323) in Lane 1: HeLa cell lysate treated with insulin, Lane 2: HepG2 cell lysate treated with insulin, Lane 3: Raw264.7 cell lysate treated with insulin. Samples were incubated with Phospho-IRS1 (Ser323) polyclonal antibody (Product # PA5-36739) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-IRS1 pSer323 using Phospho-IRS1 pSer323 polyclonal antibody (Product # PA5-36739) at a dilution of 1:500. Lane 1: Hela cell lysate treated with insulin, Lane 2: HepG2 cell lysate treated with insulin, Lane 3: Raw264.7 cell lysate treated with insulin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-IRS1 pSer323 in paraffin-embedded human breast carcinoma using Phospho-IRS1 pSer323 polyclonal antibody (Product # PA5-36739) at a dilution of 1:100.