Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-36828 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-IRS1 (Ser639) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 132 and 180 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-IRS1 (Ser639) in Lane 1: HEK293T cell lysate treated with insulin (100 nM,15mins), Lane 2: sp2/0 cell lysate treated with insulin (100 nM,15mins), Lane 3: PC12 cell lysate treated with insulin (100 nM,15mins). Samples were incubated with Phospho-IRS1 (Ser639) polyclonal antibody (Product # PA5-36828) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-IRS1 pSer639 using Phospho-IRS1 pSer639 polyclonal antibody (Product # PA5-36828) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with insulin (100nM, 15min), Lane 2: sp2/0 cell lysate treated with insulin (100nM, 15min), Lane 3: PC12 cell lysate treated with insulin (100nM, 15min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-IRS1 (Ser639) in paraffin-embedded human breast carcinoma tissue. Samples were incubated with Phospho-IRS1 (Ser639) polyclonal antibody (Product # PA5-36828) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-IRS1 (Ser639) in paraffin-embedded human breast carcinoma tissue. Samples were incubated with Phospho-IRS1 (Ser639) polyclonal antibody (Product # PA5-36828) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-IRS1 pSer639 in paraffin-embedded human breast carcinoma using Phospho-IRS1 pSer639 polyclonal antibody (Product # PA5-36828) at a dilution of 1:100.