710781

antibody from Invitrogen Antibodies

Targeting: IRS1 HIRS-1

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

710781

Provider

Invitrogen Antibodies

Product name

Phospho-IRS1 (Ser636, Ser639) Recombinant Polyclonal Antibody (25HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Pig, Cat and Bovine.

Antibody clone number

25HCLC

Concentration

0.5 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, Insulin (200 ng/mL, 10 minutes) treated MCF-7 cells were fixed and permeabilized for detection of endogenous IRSpS636/639 using Anti-IRSpS636/639 Recombinant Rabbit Polyclonal Antibody (Product # 710781, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of IRSpS636/639 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of IRSpS636/639. Panel e) shows untreated cells with no signal. Panel f) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.