MA5-36222

antibody from Invitrogen Antibodies

Targeting: IRS1 HIRS-1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-36222

Provider

Invitrogen Antibodies

Product name

IRS1 Monoclonal Antibody (10I3)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: human A549 whole cell, human T-47D whole cell, human Caco-2 whole cell, human SW620 whole cell, human Hela whole cell, human Raji whole cell. IHC: human colon cancer tissue, human lung cancer tissue, human placenta tissue. ICC/IF: MCF7 cell. Flow: PC-3 cell, U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

10I3

Vial size

100 μg

Concentration

500 μg/mL

Storage

-20°C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of IRS1 in MCF7 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in IRS1 monoclonal antibody (Product # MA5-36222) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of IRS1 in MCF7 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in IRS1 monoclonal antibody (Product # MA5-36222) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IRS1 in paraffin-embedded human placenta tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in IRS1 monoclonal antibody (Product # MA5-36222) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-mouse IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IRS1 in paraffin-embedded human lung cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in IRS1 monoclonal antibody (Product # MA5-36222) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-mouse IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IRS1 in paraffin-embedded human colon cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in IRS1 monoclonal antibody (Product # MA5-36222) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-mouse IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of IRS1 in U20S cells. Samples were blocked with 10% normal goat serum and incubated in IRS1 monoclonal antibody (Product # MA5-36222) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was mouse IgG (1 µg/1x10^6) and unlabeled sample (Red line).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of IRS1 in PC-3 cells. Samples were blocked with 10% normal goat serum and incubated in IRS1 monoclonal antibody (Product # MA5-36222) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was mouse IgG (1 µg/1x10^6) and unlabeled sample (Red line).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of IRS1 in PC-3 cells. Samples were blocked with 10% normal goat serum and incubated in IRS1 monoclonal antibody (Product # MA5-36222) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was mouse IgG (1 µg/1x10^6) and unlabeled sample (Red line).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of IRS1 in U20S cells. Samples were blocked with 10% normal goat serum and incubated in IRS1 monoclonal antibody (Product # MA5-36222) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was mouse IgG (1 µg/1x10^6) and unlabeled sample (Red line).