PA1-1055

antibody from Invitrogen Antibodies

Targeting: IRS1 HIRS-1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-1055

Provider

Invitrogen Antibodies

Product name

Phospho-IRS1 (Ser636) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-1055 detects phospho-IRS-1 (Ser636) in mouse and human cells. PA1-1055 has been successfully used in WB procedures. By Western blot, this antibody detects a ~170 kDa protein representing phospho-IRS-1 (Ser636) from 3T3 L1 cells treated with insulin for 10 minutes. The PA1-1055 immunogen is a synthetic phosphopeptide corrsponding to residues D(631) Y M P M (pS) P K S V S A P Q Q I(646) of human IRS-1. The peptide sequence is 100% conserved in rats, and mice. This peptide (Cat. # PEP-185) is available for use in neutralization and control experiments.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

The hyperglycemia-induced inflammatory response in adipocytes: the role of reactive oxygen species.

Lin Y, Berg AH, Iyengar P, Lam TK, Giacca A, Combs TP, Rajala MW, Du X, Rollman B, Li W, Hawkins M, Barzilai N, Rhodes CJ, Fantus IG, Brownlee M, Scherer PE
The Journal of biological chemistry 2005 Feb 11;280(6):4617-26

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot detection of phospho-IRS-1 (Ser636) from 3T3 L1 cells treated with insulin for 10 minutes.