Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA1-1060 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-IRS1 (Tyr1179) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-1060 detects phosphorylated IRS-1 (Tyr1173) in mouse and human cells. PA1-1060 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~170 kDa protein representing phospho-IRS-1 (Tyr1173) from 3T3 L1 cells treated with insulin. The PA1-1060 immunogen is a synthetic phosphopeptide corresponding to residues L(1173) E N G L N (pY) I D L D L V K(1186) of human IRS-1. This immunogenic sequence is 100% conserved in humans and chickens and 85% and 87% conserved in rat and mouse, respectively. The conserved phosphorylation site corresponds to residue Y1179 in human, Y1177 in chicken, Y1173 in mouse, and Y1172 in rat. This peptide (Cat. # PEP-205) is available for use in neutralization and control experiments.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot detection of phospho-IRS-1 (Tyr1173) from lysate from insulin-treated 3T3 L1 cells using Product # PA1-1060.