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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- NBP1-78078 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-78078, RRID:AB_11020744
- Product name
- Rabbit Polyclonal IRS1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is directed against the phosphorylated form of human IRS1 protein at the pS307 residue.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Dipeptidyl peptidase-4 impairs insulin signaling and promotes lipid accumulation in hepatocytes.
Rufinatscha K, Radlinger B, Dobner J, Folie S, Bon C, Profanter E, Ress C, Salzmann K, Staudacher G, Tilg H, Kaser S
Biochemical and biophysical research communications 2017 Apr 1;485(2):366-371
Biochemical and biophysical research communications 2017 Apr 1;485(2):366-371
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRS1 [p Ser307] Antibody [NBP1-78078] - Band at ~180 kDa believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate. The pronounced staining of the band at 180 kDa is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction. The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide. (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
