AF3978

antibody from R&D Systems

Targeting: IRS1 HIRS-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3978

Provider

R&D Systems

Product name

Human/Mouse/Rat IRS1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human, mouse, and rat IRS1 in Western blots.

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P35568


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human, Mouse, and Rat IRS1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat IRS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3978) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IRS1 at approximately 170 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

IRS1 in Human Liver Cancer Tissue. IRS1 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human/Mouse/Rat IRS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3978) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.