Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-82001 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-82001, RRID:AB_1145958
- Product name
- Rabbit Polyclonal IRS1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. IRS-1 (specific to a.a. 636) detects endogenous levels of total IRS-1 protein.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references NCS-1 Deficiency Is Associated With Obesity and Diabetes Type 2 in Mice.
MicroRNA 7 Impairs Insulin Signaling and Regulates Aβ Levels through Posttranscriptional Regulation of the Insulin Receptor Substrate 2, Insulin Receptor, Insulin-Degrading Enzyme, and Liver X Receptor Pathway.
Impact of heat stress during the follicular phase on porcine ovarian steroidogenic and phosphatidylinositol-3 signaling.
Ratai O, Hermainski J, Ravichandran K, Pongs O
Frontiers in molecular neuroscience 2019;12:78
Frontiers in molecular neuroscience 2019;12:78
MicroRNA 7 Impairs Insulin Signaling and Regulates Aβ Levels through Posttranscriptional Regulation of the Insulin Receptor Substrate 2, Insulin Receptor, Insulin-Degrading Enzyme, and Liver X Receptor Pathway.
Fernández-de Frutos M, Galán-Chilet I, Goedeke L, Kim B, Pardo-Marqués V, Pérez-García A, Herrero JI, Fernández-Hernando C, Kim J, Ramírez CM
Molecular and cellular biology 2019 Nov 15;39(22)
Molecular and cellular biology 2019 Nov 15;39(22)
Impact of heat stress during the follicular phase on porcine ovarian steroidogenic and phosphatidylinositol-3 signaling.
Dickson MJ, Hager CL, Al-Shaibi A, Thomas PQ, Baumgard LH, Ross JW, Keating AF
Journal of animal science 2018 Jun 4;96(6):2162-2174
Journal of animal science 2018 Jun 4;96(6):2162-2174
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRS1 Antibody [NB100-82001] - Analysis of extracts from 293 cells using IRS-1 (Ab-636) antibody
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRS1 Antibody [NB100-82001] - Analysis of extracts from 293 cells treated with insulin (100nM, 30min) or PMA (0.2nM, 15min) using IRS-1 (Ab-636) antibody (Lane 1, 2 and 3) and IRS-1 (phospho-Ser636) antibody (Lane 4, 5 and 6).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IRS1 Antibody [NB100-82001] - Analysis of breast carcinoma tissue, using IRS-1 (Ab-636) antibody.