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Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Chromatin Immunoprecipitation [1]
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- Product number
- NBP2-22439 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal JunD Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200ug
- Concentration
- LYOPH
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Chromatin Immunoprecipitation: JunD Antibody [NBP2-22439] - Analysis of JunD was performed using cross-linked chromatin from 1x10^6 HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes. IP was performed using a multiplex microplate Matrix ChIP assay with 1.0ul/100ul well volume of a JunD polyclonal antibody. Chromatin aliquots from ~1x10^5 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify exon-1 or exon-28 of the LAMC1 gene. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of ChIP is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the rat LAMC1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by LAMC1 primers are represented by black bars. Data courtesy of the Innovators Program.
