AF5526

antibody from Novus Biologicals

Targeting: JUND AP-1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5526

Provider

Novus Biologicals

Product name

Goat Polyclonal JunD Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human and mouse JunD in Western blots.

Reactivity

Human, Mouse

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse JunD by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, NIH-3T3 mouse embryonic fibroblast cell line, and MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse JunD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5526) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for JunD at approximately 40 and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.