RQ4433

antibody from NSJ Bioreagents

Targeting: JUND AP-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ4433

Provider

NSJ Bioreagents

Product name

JUND Antibody

Antibody type

Polyclonal

Description

This highly specific JUND antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the JUND antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) placenta and 2) Jurkat lysate with JUND antibody at 0.5ug/ml. Predicted molecular weight: ~39 kDa (JUND-L), 34 kDa (JUND-S). (1)

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat spleen, 2) rat lung, 3) rat testis, 4) mouse spleen, 5) mouse lung and 6) mouse testis lysate with JUND antibody at 0.5ug/ml. Predicted molecular weight: ~39 kDa (JUND-L), 34 kDa (JUND-S). (1)

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HEL, 2) human HepG2, 3) human K562, 4) human MCF7, 5) human 293T, 6) human SH-SY5Y, 7) human U-2 OS, 8) human U-251, 9) rat lung and 10) mouse lung tissue lysate with JUND antibody. Predicted molecular weight: ~39 kDa (JUND-L), 34 kDa (JUND-S). (1)

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human renal cell carcinoma tissue with JUND antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human appendix adenocarcinoma tissue with JUND antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human K562 cells with JUND antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= JUND antibody.