GTX103292

antibody from GeneTex

Targeting: LMNB1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX103292

Provider

GeneTex

Proper citation

GeneTex Cat#GTX103292, RRID:AB_1950820

Product name

Lamin B1 antibody

Antibody type

Polyclonal

Reactivity

Human, Hamster

Host

Rabbit

References

Submitted references

E74-Like Factor (ELF3) and Leptin, a Novel Loop Between Obesity and Inflammation Perpetuating a Pro-Catabolic State in Cartilage.

Conde J, Otero M, Scotece M, Abella V, Gómez R, López V, Pino J, Mera A, Goldring MB, Gualillo O
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(6):2401-2410

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Nano-Diamino-Tetrac (NDAT) Enhances Resveratrol-Induced Antiproliferation by Action on the RRM2 Pathway in Colorectal Cancers.

Nana AW, Wu SY, Yang YS, Chin YT, Cheng TM, Ho Y, Li WS, Liao YM, Chen YR, Shih YJ, Liu YR, Pedersen J, Incerpi S, Hercbergs A, Liu LF, Whang-Peng J, Davis PJ, Lin HY
Hormones & cancer 2018 Oct;9(5):349-360

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Nano-diamino-tetrac (NDAT) inhibits PD-L1 expression which is essential for proliferation in oral cancer cells.

Lin SJ, Chin YT, Ho Y, Chou SY, Sh Yang YC, Nana AW, Su KW, Lim YT, Wang K, Lee SY, Shih YJ, Chen YR, Whang-Peng J, Davis PJ, Lin HY, Fu E
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2018 Oct;120:1-11

---

Thyroxine inhibits resveratrol-caused apoptosis by PD-L1 in ovarian cancer cells.

Chin YT, Wei PL, Ho Y, Nana AW, Changou CA, Chen YR, Yang YS, Hsieh MT, Hercbergs A, Davis PJ, Shih YJ, Lin HY
Endocrine-related cancer 2018 May;25(5):533-545

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Thyroid Hormone Promotes β-Catenin Activation and Cell Proliferation in Colorectal Cancer.

Lee YS, Chin YT, Shih YJ, Nana AW, Chen YR, Wu HC, Yang YSH, Lin HY, Davis PJ
Hormones & cancer 2018 Jun;9(3):156-165

---

Scutellaria baicalensis regulates FFA metabolism to ameliorate NAFLD through the AMPK-mediated SREBP signaling pathway.

Chen Q, Liu M, Yu H, Li J, Wang S, Zhang Y, Qiu F, Wang T
Journal of natural medicines 2018 Jun;72(3):655-666

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Resveratrol induces sumoylated COX-2-dependent anti-proliferation in human prostate cancer LNCaP cells.

Cheng TM, Chin YT, Ho Y, Chen YR, Yang YN, Yang YC, Shih YJ, Lin TI, Lin HY, Davis PJ
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2018 Feb;112:67-75

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Proteomic analysis of honokiol-induced cytotoxicity in thyroid cancer cells.

Chou HC, Lu CH, Su YC, Lin LH, Yu HI, Chuang HH, Tsai YT, Liao EC, Wei YS, Yang YT, Chien YA, Yu XR, Lee YR, Chan HL
Life sciences 2018 Aug 15;207:184-204

---

3,4-Dihydroxybenzalactone Suppresses Human Non-Small Cell Lung Carcinoma Cells Metastasis via Suppression of Epithelial to Mesenchymal Transition, ROS-Mediated PI3K/AKT/MAPK/MMP and NFκB Signaling Pathways.

Chao W, Deng JS, Li PY, Liang YC, Huang GJ
Molecules (Basel, Switzerland) 2017 Mar 28;22(4)

---

Human cactin interacts with DHX8 and SRRM2 to assure efficient pre-mRNA splicing and sister chromatid cohesion.

Zanini IM, Soneson C, Lorenzi LE, Azzalin CM
Journal of cell science 2017 Feb 15;130(4):767-778

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Expression and Activity of Breast Cancer Resistance Protein (BCRP/ABCG2) in Human Distal Lung Epithelial Cells In Vitro.

Nickel S, Selo MA, Fallack J, Clerkin CG, Huwer H, Schneider-Daum N, Lehr CM, Ehrhardt C
Pharmaceutical research 2017 Dec;34(12):2477-2487

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Reactive oxygen species-mediated switching expression of MMP-3 in stromal fibroblasts and cancer cells during prostate cancer progression.

Hsieh CL, Liu CM, Chen HA, Yang ST, Shigemura K, Kitagawa K, Yamamichi F, Fujisawa M, Liu YR, Lee WH, Chen KC, Shen CN, Lin CC, Chung LWK, Sung SY
Scientific reports 2017 Aug 22;7(1):9065

---

Protective Effect of Caffeic Acid Derivatives on tert-Butyl Hydroperoxide-Induced Oxidative Hepato-Toxicity and Mitochondrial Dysfunction in HepG2 Cells.

Tsai TH, Yu CH, Chang YP, Lin YT, Huang CJ, Kuo YH, Tsai PJ
Molecules (Basel, Switzerland) 2017 Apr 28;22(5)

---

Crosstalk between integrin αvβ3 and ERα contributes to thyroid hormone-induced proliferation of ovarian cancer cells.

Hsieh MT, Wang LM, Changou CA, Chin YT, Yang YSH, Lai HY, Lee SY, Yang YN, Whang-Peng J, Liu LF, Lin HY, Mousa SA, Davis PJ
Oncotarget 2017 Apr 11;8(15):24237-24249

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Characterization of MUDENG, a novel anti-apoptotic protein.

Choi JH, Lim JB, Wickramanayake DD, Wagley Y, Kim J, Lee HC, Seo HG, Kim TH, Oh JW
Oncogenesis 2016 May 2;5:e221

---

SERPINE2 Inhibits IL-1α-Induced MMP-13 Expression in Human Chondrocytes: Involvement of ERK/NF-κB/AP-1 Pathways.

Santoro A, Conde J, Scotece M, Abella V, Lois A, Lopez V, Pino J, Gomez R, Gomez-Reino JJ, Gualillo O
PloS one 2015;10(8):e0135979

---

Amphotericin B and anidulafungin directly interact with DNA and induce oxidative damage in the mammalian genome.

Mandal SM, Chakraborty A, Hossain M, Mahata D, Porto WF, Chakraborty R, Mukhopadhyay CK, Franco OL, Hazra TK, Basak A
Molecular bioSystems 2015 Sep;11(9):2551-9

---

Regulation of the tumor suppressor FOXO3 by the thromboxane-A2 receptors in urothelial cancer.

Sobolesky PM, Halushka PV, Garrett-Mayer E, Smith MT, Moussa O
PloS one 2014;9(9):e107530

---

RNaseH1 regulates TERRA-telomeric DNA hybrids and telomere maintenance in ALT tumour cells.

Arora R, Lee Y, Wischnewski H, Brun CM, Schwarz T, Azzalin CM
Nature communications 2014 Oct 21;5:5220

---

Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells.

Lee JC, Tseng CK, Young KC, Sun HY, Wang SW, Chen WC, Lin CK, Wu YH
British journal of pharmacology 2014 Jan;171(1):237-52

---

Regulation of estrogen receptor α function in oral squamous cell carcinoma cells by FAK signaling.

Chang YL, Hsu YK, Wu TF, Huang CM, Liou LY, Chiu YW, Hsiao YH, Luo FJ, Yuan TC
Endocrine-related cancer 2014 Aug;21(4):555-65

---

Nuclear export and import of human hepatitis B virus capsid protein and particles.

Li HC, Huang EY, Su PY, Wu SY, Yang CC, Lin YS, Chang WC, Shih C
PLoS pathogens 2010 Oct 28;6(10):e1001162

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE GTX103292 diluted at 1:5000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample(30 ug whole cell lysate)A:A431(GTX27909)B:H1299C:HeLa S3(GTX14654)7.5% SDS PAGEGTX103292 diluted at 1:5000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Lamin B1 antibody detects Lamin B1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody (GTX103292) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and Lamin B1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody (GTX103292) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and Lamin B1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody (GTX103292) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of Lamin B1 protein from 293T whole cell extracts using 5 £gg of Lamin B1 antibody (GTX103292).Western blot analysis was performed using Lamin B1 antibody (GTX103292).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Lamin B1 antibody(GTX103292) antibody at 1:250 dilution.