Antibody data
- Antibody Data
- Antigen structure
- References [61]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
- Other assay [22]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 33-2000 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Lamin B1 Monoclonal Antibody (L-5)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- L-5
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Nucleolar localization of the ErbB3 receptor as a new target in glioblastoma.
ERAD components Derlin-1 and Derlin-2 are essential for postnatal brain development and motor function.
Tight junction protein ZO-2 modulates the nuclear accumulation of transcription factor TEAD.
Regulation of dimethylarginine dimethylaminohydrolase 2 expression by NF-κB acetylation.
Cancer Stem Cell-Inducing Media Activates Senescence Reprogramming in Fibroblasts.
Nelfinavir Inhibits the TCF11/Nrf1-Mediated Proteasome Recovery Pathway in Multiple Myeloma.
HCV and flaviviruses hijack cellular mechanisms for nuclear STAT2 degradation: Up-regulation of PDLIM2 suppresses the innate immune response.
Activation of the Ca(2+) sensing receptor and the PKC/WNK4 downstream signaling cascade induces incorporation of ZO-2 to tight junctions and its separation from 14-3-3.
Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.
Anti-Inflammatory Effect of Melittin on Porphyromonas Gingivalis LPS-Stimulated Human Keratinocytes.
Epigenetic regulator CXXC5 recruits DNA demethylase Tet2 to regulate TLR7/9-elicited IFN response in pDCs.
SUMOylation regulates the intracellular fate of ZO-2.
Monocytes of patients with amyotrophic lateral sclerosis linked to gene mutations display altered TDP-43 subcellular distribution.
Synergistic activity of Card11 mutant and Bcl6 in the development of diffuse large B-cell lymphoma in a mouse model.
Bee Venom Inhibits Porphyromonas gingivalis Lipopolysaccharides-Induced Pro-Inflammatory Cytokines through Suppression of NF-κB and AP-1 Signaling Pathways.
Tumour suppressor death-associated protein kinase targets cytoplasmic HIF-1α for Th17 suppression.
A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity.
Nrf2-Mediated Regulation of Skeletal Muscle Glycogen Metabolism.
A Cytosolic Multiprotein Complex Containing p85α Is Required for β-Catenin Activation in Colitis and Colitis-associated Cancer.
Rhinovirus 16 2A Protease Affects Nuclear Localization of 3CD during Infection.
Preadipocyte factor 1 induces pancreatic ductal cell differentiation into insulin-producing cells.
The Soluble Heparin-Binding EGF-Like Growth Factor Stimulates EGF Receptor Trafficking to the Nucleus.
Snai1 promotes ESC exit from the pluripotency by direct repression of self-renewal genes.
mTOR regulates TLR-induced c-fos and Th1 responses to HBV and HCV vaccines.
Defects of Lipid Synthesis Are Linked to the Age-Dependent Demyelination Caused by Lamin B1 Overexpression.
Tenfibgen ligand nanoencapsulation delivers bi-functional anti-CK2 RNAi oligomer to key sites for prostate cancer targeting using human xenograft tumors in mice.
Immunoneutralization of endogenous aminoprocalcitonin attenuates sepsis-induced acute lung injury and mortality in rats.
Growth differentiation factor-5 promotes brown adipogenesis in systemic energy expenditure.
Inositol Polyphosphate-5-Phosphatase F (INPP5F) inhibits STAT3 activity and suppresses gliomas tumorigenicity.
Cell Fractionation and Quantitative Analysis of HIV-1 Reverse Transcription in Target Cells.
Interferon β induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in mice.
Heart-specific stiffening in early embryos parallels matrix and myosin expression to optimize beating.
The intracellular fate of zonula occludens 2 is regulated by the phosphorylation of SR repeats and the phosphorylation/O-GlcNAcylation of S257.
The piggyBac transposon displays local and distant reintegration preferences and can cause mutations at noncanonical integration sites.
The imprinted NPAP1/C15orf2 gene in the Prader-Willi syndrome region encodes a nuclear pore complex associated protein.
Maxacalcitol ameliorates tubulointerstitial fibrosis in obstructed kidneys by recruiting PPM1A/VDR complex to pSmad3.
miR-218 directs a Wnt signaling circuit to promote differentiation of osteoblasts and osteomimicry of metastatic cancer cells.
Kaposi's sarcoma-associated herpesvirus-encoded latency-associated nuclear antigen reduces interleukin-8 expression in endothelial cells and impairs neutrophil chemotaxis by degrading nuclear p65.
Protection against 2-chloroethyl ethyl sulfide (CEES)-induced cytotoxicity in human keratinocytes by an inducer of the glutathione detoxification pathway.
Systemic administration of antisense oligonucleotides simultaneously targeting CK2α and α' subunits reduces orthotopic xenograft prostate tumors in mice.
Nuclear lamins are differentially expressed in retinal neurons of the adult rat retina.
Dual regulation of the transcriptional activity of Nrf1 by β-TrCP- and Hrd1-dependent degradation mechanisms.
Cytoplasmic accumulation of TDP-43 in circulating lymphomonocytes of ALS patients with and without TARDBP mutations.
In CD28-costimulated human naïve CD4+ T cells, I-κB kinase controls the expression of cell cycle regulatory proteins via interleukin-2-independent mechanisms.
The BRCA-1 binding protein BRAP2 is a novel, negative regulator of nuclear import of viral proteins, dependent on phosphorylation flanking the nuclear localization signal.
TDP-43 redistribution is an early event in sporadic amyotrophic lateral sclerosis.
Enhanced hypothalamic leptin signaling in mice lacking dopamine D2 receptors.
TNFAIP3/A20 functions as a novel tumor suppressor gene in several subtypes of non-Hodgkin lymphomas.
JNJ-26481585, a novel "second-generation" oral histone deacetylase inhibitor, shows broad-spectrum preclinical antitumoral activity.
Embryonic overexpression of Galgt2 inhibits skeletal muscle growth via activation of myostatin signaling.
Chronic endoplasmic reticulum stress activates unfolded protein response in arterial endothelium in regions of susceptibility to atherosclerosis.
Carbon monoxide produced by heme oxygenase-1 in response to nitrosative stress induces expression of glutamate-cysteine ligase in PC12 cells via activation of phosphatidylinositol 3-kinase and Nrf2 signaling.
Capsaicin induces heme oxygenase-1 expression in HepG2 cells via activation of PI3K-Nrf2 signaling: NAD(P)H:quinone oxidoreductase as a potential target.
Specific role of Chk1 phosphorylations in cell survival and checkpoint activation.
A proapoptotic function of p21 in differentiating granulocytes.
Point mutation in AML1 disrupts subnuclear targeting, prevents myeloid differentiation, and effects a transformation-like phenotype.
Two distinct Fas-activated signaling pathways revealed by an antitumor drug D609.
Characterization of the tight junction protein ZO-2 localized at the nucleus of epithelial cells.
The tight junction protein ZO-2 associates with Jun, Fos and C/EBP transcription factors in epithelial cells.
The tight junction protein ZO-2 associates with Jun, Fos and C/EBP transcription factors in epithelial cells.
Expression of lamins depends on epidermal differentiation and transformation.
Tagliaferro M, Rosa P, Bellenchi GC, Bastianelli D, Trotta R, Tito C, Fazi F, Calogero A, Ponti D
BMC molecular and cell biology 2022 Mar 7;23(1):13
BMC molecular and cell biology 2022 Mar 7;23(1):13
ERAD components Derlin-1 and Derlin-2 are essential for postnatal brain development and motor function.
Sugiyama T, Murao N, Kadowaki H, Takao K, Miyakawa T, Matsushita Y, Katagiri T, Futatsugi A, Shinmyo Y, Kawasaki H, Sakai J, Shiomi K, Nakazato M, Takeda K, Mikoshiba K, Ploegh HL, Ichijo H, Nishitoh H
iScience 2021 Jul 23;24(7):102758
iScience 2021 Jul 23;24(7):102758
Tight junction protein ZO-2 modulates the nuclear accumulation of transcription factor TEAD.
Gallego-Gutiérrez H, González-González L, Ramírez-Martínez L, López-Bayghen E, González-Mariscal L
Molecular biology of the cell 2021 Jul 15;32(15):1347-1358
Molecular biology of the cell 2021 Jul 15;32(15):1347-1358
Regulation of dimethylarginine dimethylaminohydrolase 2 expression by NF-κB acetylation.
Li J, Sun L, Li Y
Experimental and therapeutic medicine 2021 Feb;21(2):114
Experimental and therapeutic medicine 2021 Feb;21(2):114
Cancer Stem Cell-Inducing Media Activates Senescence Reprogramming in Fibroblasts.
Perrigue PM, Rakoczy M, Pawlicka KP, Belter A, Giel-Pietraszuk M, Naskręt-Barciszewska M, Barciszewski J, Figlerowicz M
Cancers 2020 Jun 30;12(7)
Cancers 2020 Jun 30;12(7)
Nelfinavir Inhibits the TCF11/Nrf1-Mediated Proteasome Recovery Pathway in Multiple Myeloma.
Fassmannová D, Sedlák F, Sedláček J, Špička I, Grantz Šašková K
Cancers 2020 Apr 25;12(5)
Cancers 2020 Apr 25;12(5)
HCV and flaviviruses hijack cellular mechanisms for nuclear STAT2 degradation: Up-regulation of PDLIM2 suppresses the innate immune response.
Joyce MA, Berry-Wynne KM, Dos Santos T, Addison WR, McFarlane N, Hobman T, Tyrrell DL
PLoS pathogens 2019 Aug;15(8):e1007949
PLoS pathogens 2019 Aug;15(8):e1007949
Activation of the Ca(2+) sensing receptor and the PKC/WNK4 downstream signaling cascade induces incorporation of ZO-2 to tight junctions and its separation from 14-3-3.
Amaya E, Alarcón L, Martín-Tapia D, Cuellar-Pérez F, Cano-Cortina M, Ortega-Olvera JM, Cisneros B, Rodriguez AJ, Gamba G, González-Mariscal L
Molecular biology of the cell 2019 Aug 15;30(18):2377-2398
Molecular biology of the cell 2019 Aug 15;30(18):2377-2398
Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.
Maclean KN, Jiang H, Aivazidis S, Kim E, Shearn CT, Harris PS, Petersen DR, Allen RH, Stabler SP, Roede JR
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Mar;32(3):1265-1280
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Mar;32(3):1265-1280
Anti-Inflammatory Effect of Melittin on Porphyromonas Gingivalis LPS-Stimulated Human Keratinocytes.
Kim WH, An HJ, Kim JY, Gwon MG, Gu H, Jeon M, Kim MK, Han SM, Park KK
Molecules (Basel, Switzerland) 2018 Feb 5;23(2)
Molecules (Basel, Switzerland) 2018 Feb 5;23(2)
Epigenetic regulator CXXC5 recruits DNA demethylase Tet2 to regulate TLR7/9-elicited IFN response in pDCs.
Ma S, Wan X, Deng Z, Shi L, Hao C, Zhou Z, Zhou C, Fang Y, Liu J, Yang J, Chen X, Li T, Zang A, Yin S, Li B, Plumas J, Chaperot L, Zhang X, Xu G, Jiang L, Shen N, Xiong S, Gao X, Zhang Y, Xiao H
The Journal of experimental medicine 2017 May 1;214(5):1471-1491
The Journal of experimental medicine 2017 May 1;214(5):1471-1491
SUMOylation regulates the intracellular fate of ZO-2.
Wetzel F, Mittag S, Cano-Cortina M, Wagner T, Krämer OH, Niedenthal R, Gonzalez-Mariscal L, Huber O
Cellular and molecular life sciences : CMLS 2017 Jan;74(2):373-392
Cellular and molecular life sciences : CMLS 2017 Jan;74(2):373-392
Monocytes of patients with amyotrophic lateral sclerosis linked to gene mutations display altered TDP-43 subcellular distribution.
De Marco G, Lomartire A, Calvo A, Risso A, De Luca E, Mostert M, Mandrioli J, Caponnetto C, Borghero G, Manera U, Canosa A, Moglia C, Restagno G, Fini N, Tarella C, Giordana MT, Rinaudo MT, Chiò A
Neuropathology and applied neurobiology 2017 Feb;43(2):133-153
Neuropathology and applied neurobiology 2017 Feb;43(2):133-153
Synergistic activity of Card11 mutant and Bcl6 in the development of diffuse large B-cell lymphoma in a mouse model.
Takahara T, Matsuo K, Seto M, Nakamura S, Tsuzuki S
Cancer science 2016 Nov;107(11):1572-1580
Cancer science 2016 Nov;107(11):1572-1580
Bee Venom Inhibits Porphyromonas gingivalis Lipopolysaccharides-Induced Pro-Inflammatory Cytokines through Suppression of NF-κB and AP-1 Signaling Pathways.
Kim WH, An HJ, Kim JY, Gwon MG, Gu H, Park JB, Sung WJ, Kwon YC, Park KD, Han SM, Park KK
Molecules (Basel, Switzerland) 2016 Nov 10;21(11)
Molecules (Basel, Switzerland) 2016 Nov 10;21(11)
Tumour suppressor death-associated protein kinase targets cytoplasmic HIF-1α for Th17 suppression.
Chou TF, Chuang YT, Hsieh WC, Chang PY, Liu HY, Mo ST, Hsu TS, Miaw SC, Chen RH, Kimchi A, Lai MZ
Nature communications 2016 Jun 17;7:11904
Nature communications 2016 Jun 17;7:11904
A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity.
Fagnocchi L, Cherubini A, Hatsuda H, Fasciani A, Mazzoleni S, Poli V, Berno V, Rossi RL, Reinbold R, Endele M, Schroeder T, Rocchigiani M, Szkarłat Ż, Oliviero S, Dalton S, Zippo A
Nature communications 2016 Jun 15;7:11903
Nature communications 2016 Jun 15;7:11903
Nrf2-Mediated Regulation of Skeletal Muscle Glycogen Metabolism.
Uruno A, Yagishita Y, Katsuoka F, Kitajima Y, Nunomiya A, Nagatomi R, Pi J, Biswal SS, Yamamoto M
Molecular and cellular biology 2016 Jun 1;36(11):1655-72
Molecular and cellular biology 2016 Jun 1;36(11):1655-72
A Cytosolic Multiprotein Complex Containing p85α Is Required for β-Catenin Activation in Colitis and Colitis-associated Cancer.
Goretsky T, Bradford EM, Ryu H, Tahir M, Moyer MP, Gao T, Li L, Barrett TA
The Journal of biological chemistry 2016 Feb 19;291(8):4166-77
The Journal of biological chemistry 2016 Feb 19;291(8):4166-77
Rhinovirus 16 2A Protease Affects Nuclear Localization of 3CD during Infection.
Walker E, Jensen L, Croft S, Wei K, Fulcher AJ, Jans DA, Ghildyal R
Journal of virology 2016 Dec 15;90(24):11032-11042
Journal of virology 2016 Dec 15;90(24):11032-11042
Preadipocyte factor 1 induces pancreatic ductal cell differentiation into insulin-producing cells.
Rhee M, Lee SH, Kim JW, Ham DS, Park HS, Yang HK, Shin JY, Cho JH, Kim YB, Youn BS, Sul HS, Yoon KH
Scientific reports 2016 Apr 5;6:23960
Scientific reports 2016 Apr 5;6:23960
The Soluble Heparin-Binding EGF-Like Growth Factor Stimulates EGF Receptor Trafficking to the Nucleus.
Korotkevych NV, Labyntsev AJ, Kolybo DV, Komisarenko SV
PloS one 2015;10(5):e0127887
PloS one 2015;10(5):e0127887
Snai1 promotes ESC exit from the pluripotency by direct repression of self-renewal genes.
Galvagni F, Lentucci C, Neri F, Dettori D, De Clemente C, Orlandini M, Anselmi F, Rapelli S, Grillo M, Borghi S, Oliviero S
Stem cells (Dayton, Ohio) 2015 Mar;33(3):742-50
Stem cells (Dayton, Ohio) 2015 Mar;33(3):742-50
mTOR regulates TLR-induced c-fos and Th1 responses to HBV and HCV vaccines.
He L, Zang A, Du M, Ma D, Yuan C, Zhou C, Mu J, Shi H, Li D, Huang X, Deng Q, Xiao J, Yan H, Hui L, Lan K, Xiong S, Li X, Huang Z, Xiao H
Virologica Sinica 2015 Jun;30(3):174-89
Virologica Sinica 2015 Jun;30(3):174-89
Defects of Lipid Synthesis Are Linked to the Age-Dependent Demyelination Caused by Lamin B1 Overexpression.
Rolyan H, Tyurina YY, Hernandez M, Amoscato AA, Sparvero LJ, Nmezi BC, Lu Y, Estécio MR, Lin K, Chen J, He RR, Gong P, Rigatti LH, Dupree J, Bayır H, Kagan VE, Casaccia P, Padiath QS
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Aug 26;35(34):12002-17
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Aug 26;35(34):12002-17
Tenfibgen ligand nanoencapsulation delivers bi-functional anti-CK2 RNAi oligomer to key sites for prostate cancer targeting using human xenograft tumors in mice.
Trembley JH, Unger GM, Korman VL, Abedin MJ, Nacusi LP, Vogel RI, Slaton JW, Kren BT, Ahmed K
PloS one 2014;9(10):e109970
PloS one 2014;9(10):e109970
Immunoneutralization of endogenous aminoprocalcitonin attenuates sepsis-induced acute lung injury and mortality in rats.
Tavares E, Maldonado R, Miñano FJ
The American journal of pathology 2014 Nov;184(11):3069-83
The American journal of pathology 2014 Nov;184(11):3069-83
Growth differentiation factor-5 promotes brown adipogenesis in systemic energy expenditure.
Hinoi E, Nakamura Y, Takada S, Fujita H, Iezaki T, Hashizume S, Takahashi S, Odaka Y, Watanabe T, Yoneda Y
Diabetes 2014 Jan;63(1):162-75
Diabetes 2014 Jan;63(1):162-75
Inositol Polyphosphate-5-Phosphatase F (INPP5F) inhibits STAT3 activity and suppresses gliomas tumorigenicity.
Kim HS, Li A, Ahn S, Song H, Zhang W
Scientific reports 2014 Dec 5;4:7330
Scientific reports 2014 Dec 5;4:7330
Cell Fractionation and Quantitative Analysis of HIV-1 Reverse Transcription in Target Cells.
Shah VB, Aiken C
Bio-protocol 2013 Oct 20;3(20)
Bio-protocol 2013 Oct 20;3(20)
Interferon β induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in mice.
Chort A, Alves S, Marinello M, Dufresnois B, Dornbierer JG, Tesson C, Latouche M, Baker DP, Barkats M, El Hachimi KH, Ruberg M, Janer A, Stevanin G, Brice A, Sittler A
Brain : a journal of neurology 2013 Jun;136(Pt 6):1732-45
Brain : a journal of neurology 2013 Jun;136(Pt 6):1732-45
Heart-specific stiffening in early embryos parallels matrix and myosin expression to optimize beating.
Majkut S, Idema T, Swift J, Krieger C, Liu A, Discher DE
Current biology : CB 2013 Dec 2;23(23):2434-9
Current biology : CB 2013 Dec 2;23(23):2434-9
The intracellular fate of zonula occludens 2 is regulated by the phosphorylation of SR repeats and the phosphorylation/O-GlcNAcylation of S257.
Quiros M, Alarcón L, Ponce A, Giannakouros T, González-Mariscal L
Molecular biology of the cell 2013 Aug;24(16):2528-43
Molecular biology of the cell 2013 Aug;24(16):2528-43
The piggyBac transposon displays local and distant reintegration preferences and can cause mutations at noncanonical integration sites.
Li MA, Pettitt SJ, Eckert S, Ning Z, Rice S, Cadiñanos J, Yusa K, Conte N, Bradley A
Molecular and cellular biology 2013 Apr;33(7):1317-30
Molecular and cellular biology 2013 Apr;33(7):1317-30
The imprinted NPAP1/C15orf2 gene in the Prader-Willi syndrome region encodes a nuclear pore complex associated protein.
Neumann LC, Markaki Y, Mladenov E, Hoffmann D, Buiting K, Horsthemke B
Human molecular genetics 2012 Sep 15;21(18):4038-48
Human molecular genetics 2012 Sep 15;21(18):4038-48
Maxacalcitol ameliorates tubulointerstitial fibrosis in obstructed kidneys by recruiting PPM1A/VDR complex to pSmad3.
Inoue K, Matsui I, Hamano T, Fujii N, Shimomura A, Nakano C, Kusunoki Y, Takabatake Y, Hirata M, Nishiyama A, Tsubakihara Y, Isaka Y, Rakugi H
Laboratory investigation; a journal of technical methods and pathology 2012 Dec;92(12):1686-97
Laboratory investigation; a journal of technical methods and pathology 2012 Dec;92(12):1686-97
miR-218 directs a Wnt signaling circuit to promote differentiation of osteoblasts and osteomimicry of metastatic cancer cells.
Hassan MQ, Maeda Y, Taipaleenmaki H, Zhang W, Jafferji M, Gordon JA, Li Z, Croce CM, van Wijnen AJ, Stein JL, Stein GS, Lian JB
The Journal of biological chemistry 2012 Dec 7;287(50):42084-92
The Journal of biological chemistry 2012 Dec 7;287(50):42084-92
Kaposi's sarcoma-associated herpesvirus-encoded latency-associated nuclear antigen reduces interleukin-8 expression in endothelial cells and impairs neutrophil chemotaxis by degrading nuclear p65.
Li X, Liang D, Lin X, Robertson ES, Lan K
Journal of virology 2011 Sep;85(17):8606-15
Journal of virology 2011 Sep;85(17):8606-15
Protection against 2-chloroethyl ethyl sulfide (CEES)-induced cytotoxicity in human keratinocytes by an inducer of the glutathione detoxification pathway.
Abel EL, Bubel JD, Simper MS, Powell L, McClellan SA, Andreeff M, MacLeod MC, DiGiovanni J
Toxicology and applied pharmacology 2011 Sep 1;255(2):176-83
Toxicology and applied pharmacology 2011 Sep 1;255(2):176-83
Systemic administration of antisense oligonucleotides simultaneously targeting CK2α and α' subunits reduces orthotopic xenograft prostate tumors in mice.
Trembley JH, Unger GM, Tobolt DK, Korman VL, Wang G, Ahmad KA, Slaton JW, Kren BT, Ahmed K
Molecular and cellular biochemistry 2011 Oct;356(1-2):21-35
Molecular and cellular biochemistry 2011 Oct;356(1-2):21-35
Nuclear lamins are differentially expressed in retinal neurons of the adult rat retina.
Wakabayashi T, Mori T, Hirahara Y, Koike T, Kubota Y, Takamori Y, Yamada H
Histochemistry and cell biology 2011 Oct;136(4):427-36
Histochemistry and cell biology 2011 Oct;136(4):427-36
Dual regulation of the transcriptional activity of Nrf1 by β-TrCP- and Hrd1-dependent degradation mechanisms.
Tsuchiya Y, Morita T, Kim M, Iemura S, Natsume T, Yamamoto M, Kobayashi A
Molecular and cellular biology 2011 Nov;31(22):4500-12
Molecular and cellular biology 2011 Nov;31(22):4500-12
Cytoplasmic accumulation of TDP-43 in circulating lymphomonocytes of ALS patients with and without TARDBP mutations.
De Marco G, Lupino E, Calvo A, Moglia C, Buccinnà B, Grifoni S, Ramondetti C, Lomartire A, Rinaudo MT, Piccinini M, Giordana MT, Chiò A
Acta neuropathologica 2011 May;121(5):611-22
Acta neuropathologica 2011 May;121(5):611-22
In CD28-costimulated human naïve CD4+ T cells, I-κB kinase controls the expression of cell cycle regulatory proteins via interleukin-2-independent mechanisms.
Lupino E, Buccinnà B, Ramondetti C, Lomartire A, De Marco G, Ricotti E, Tovo PA, Rinaudo MT, Piccinini M
Immunology 2010 Oct;131(2):231-41
Immunology 2010 Oct;131(2):231-41
The BRCA-1 binding protein BRAP2 is a novel, negative regulator of nuclear import of viral proteins, dependent on phosphorylation flanking the nuclear localization signal.
Fulcher AJ, Roth DM, Fatima S, Alvisi G, Jans DA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 May;24(5):1454-66
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 May;24(5):1454-66
TDP-43 redistribution is an early event in sporadic amyotrophic lateral sclerosis.
Giordana MT, Piccinini M, Grifoni S, De Marco G, Vercellino M, Magistrello M, Pellerino A, Buccinnà B, Lupino E, Rinaudo MT
Brain pathology (Zurich, Switzerland) 2010 Mar;20(2):351-60
Brain pathology (Zurich, Switzerland) 2010 Mar;20(2):351-60
Enhanced hypothalamic leptin signaling in mice lacking dopamine D2 receptors.
Kim KS, Yoon YR, Lee HJ, Yoon S, Kim SY, Shin SW, An JJ, Kim MS, Choi SY, Sun W, Baik JH
The Journal of biological chemistry 2010 Mar 19;285(12):8905-17
The Journal of biological chemistry 2010 Mar 19;285(12):8905-17
TNFAIP3/A20 functions as a novel tumor suppressor gene in several subtypes of non-Hodgkin lymphomas.
Honma K, Tsuzuki S, Nakagawa M, Tagawa H, Nakamura S, Morishima Y, Seto M
Blood 2009 Sep 17;114(12):2467-75
Blood 2009 Sep 17;114(12):2467-75
JNJ-26481585, a novel "second-generation" oral histone deacetylase inhibitor, shows broad-spectrum preclinical antitumoral activity.
Arts J, King P, Mariën A, Floren W, Beliën A, Janssen L, Pilatte I, Roux B, Decrane L, Gilissen R, Hickson I, Vreys V, Cox E, Bol K, Talloen W, Goris I, Andries L, Du Jardin M, Janicot M, Page M, van Emelen K, Angibaud P
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Nov 15;15(22):6841-51
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Nov 15;15(22):6841-51
Embryonic overexpression of Galgt2 inhibits skeletal muscle growth via activation of myostatin signaling.
Chandraskeharan K, Martin PT
Muscle & nerve 2009 Jan;39(1):25-41
Muscle & nerve 2009 Jan;39(1):25-41
Chronic endoplasmic reticulum stress activates unfolded protein response in arterial endothelium in regions of susceptibility to atherosclerosis.
Civelek M, Manduchi E, Riley RJ, Stoeckert CJ Jr, Davies PF
Circulation research 2009 Aug 28;105(5):453-61
Circulation research 2009 Aug 28;105(5):453-61
Carbon monoxide produced by heme oxygenase-1 in response to nitrosative stress induces expression of glutamate-cysteine ligase in PC12 cells via activation of phosphatidylinositol 3-kinase and Nrf2 signaling.
Li MH, Jang JH, Na HK, Cha YN, Surh YJ
The Journal of biological chemistry 2007 Sep 28;282(39):28577-28586
The Journal of biological chemistry 2007 Sep 28;282(39):28577-28586
Capsaicin induces heme oxygenase-1 expression in HepG2 cells via activation of PI3K-Nrf2 signaling: NAD(P)H:quinone oxidoreductase as a potential target.
Joung EJ, Li MH, Lee HG, Somparn N, Jung YS, Na HK, Kim SH, Cha YN, Surh YJ
Antioxidants & redox signaling 2007 Dec;9(12):2087-98
Antioxidants & redox signaling 2007 Dec;9(12):2087-98
Specific role of Chk1 phosphorylations in cell survival and checkpoint activation.
Niida H, Katsuno Y, Banerjee B, Hande MP, Nakanishi M
Molecular and cellular biology 2007 Apr;27(7):2572-81
Molecular and cellular biology 2007 Apr;27(7):2572-81
A proapoptotic function of p21 in differentiating granulocytes.
Ghanem L, Steinman R
Leukemia research 2005 Nov;29(11):1315-23
Leukemia research 2005 Nov;29(11):1315-23
Point mutation in AML1 disrupts subnuclear targeting, prevents myeloid differentiation, and effects a transformation-like phenotype.
Vradii D, Zaidi SK, Lian JB, van Wijnen AJ, Stein JL, Stein GS
Proceedings of the National Academy of Sciences of the United States of America 2005 May 17;102(20):7174-9
Proceedings of the National Academy of Sciences of the United States of America 2005 May 17;102(20):7174-9
Two distinct Fas-activated signaling pathways revealed by an antitumor drug D609.
Zhang L, Shimizu S, Tsujimoto Y
Oncogene 2005 Apr 21;24(18):2954-62
Oncogene 2005 Apr 21;24(18):2954-62
Characterization of the tight junction protein ZO-2 localized at the nucleus of epithelial cells.
Jaramillo BE, Ponce A, Moreno J, Betanzos A, Huerta M, Lopez-Bayghen E, Gonzalez-Mariscal L
Experimental cell research 2004 Jul 1;297(1):247-58
Experimental cell research 2004 Jul 1;297(1):247-58
The tight junction protein ZO-2 associates with Jun, Fos and C/EBP transcription factors in epithelial cells.
Betanzos A, Huerta M, Lopez-Bayghen E, Azuara E, Amerena J, González-Mariscal L
Experimental cell research 2004 Jan 1;292(1):51-66
Experimental cell research 2004 Jan 1;292(1):51-66
The tight junction protein ZO-2 associates with Jun, Fos and C/EBP transcription factors in epithelial cells.
Betanzos A, Huerta M, Lopez-Bayghen E, Azuara E, Amerena J, González-Mariscal L
Experimental cell research 2004 Jan 1;292(1):51-66
Experimental cell research 2004 Jan 1;292(1):51-66
Expression of lamins depends on epidermal differentiation and transformation.
Oguchi M, Sagara J, Matsumoto K, Saida T, Taniguchi S
The British journal of dermatology 2002 Nov;147(5):853-8
The British journal of dermatology 2002 Nov;147(5):853-8
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- Western blot analysis was performed on nuclear enriched cell extracts of HeLa (Lane 1), HEK-293 (Lane 2) and K-562 (Lane 3). The blots were probed with Anti- Lamin B1 Mouse Monoclonal Antibody (Product # 33-2000, 2µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A ~ 66 kDa band corresponding to Lamin B1 was observed across all cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
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- Knockout of Lamin B1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR711025_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Lamin B1 was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) andHeLa Lamin B1 KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-Lamin B1 Monoclonal Antibody (L-5) (Product # 33-2000, 1:250 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:5,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to Lamin B1. An uncharacterized band was observed in HeLa Cas9 sample at ~45 kDa.
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- Flow cytometry analysis of Lamin B1 was done on HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Lamin B1 Mouse Monoclonal Antibody (Product # 33-2000, red histogram) or with mouse isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..
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- FIGURE 3: ZO-2 triple mutant S257E/S259E/S261E and the single mutants S257E and S257A arrive at the nucleus with a delay. (A) Percentage of cells with nuclear HA-ZO-2 as a function of time. The percentage of cells with nuclear ZO-2 was determined by immunofluorescence using an anti-HA antibody. Monolayers were fixed at the indicated times. Time 0 corresponds to 6 h after transfection. Experiments were done with cells transfected with full-length HA-ZO-2 (WT), the triple mutant S257E/S259E/S261E and single mutants S257E and S257A. Results from three independent experiments. In each experiment 100 transfected cells were evaluated per experimental point. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 with respect to ZO-2 WT as assessed by two-way ANOVA followed by Bonferroni's post hoc test. (B) Detection by Western blot of HA-ZO-2 present in plasma membrane and nuclear fractions derived from cells 24 h after being transfected with HA-ZO-2 WT or the mutants S257A and S257E. Lamin B1 and Na + -K + -ATPase beta1 subunit antibodies were used as positive controls of nuclear and plasma membrane fractions, respectively.
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- Figure 5 Proof-of-concept for therapeutic efficacy and mechanism of s50-TBG-RNAi-CK2 in orthotopic and metastatic prostate cancer xenograft tumors. ( a ) Primary tumor mass and lymph node tumor volumes are shown following s50-TBG-RNAi-CK2 treatments at 33 and 330 ng/kg compared to vehicle. Means +- SE are presented (TBG-RNAi-CK2 n = 4; Vehicle n = 5). * p
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- Figure 5 INPP5F inhibits STAT3 phosphorylation and nuclear translocation. (A) Immunoblot analysis shows that INPP5F overexpression in glioblastoma cells (GSC923, GSC1228, and U87) inhibits phosphorylation of STAT3 (Y705) and INPP5F knockdown (GSC211 and GSC827) enhances its phosphorylation. (B) Immunoblot analysis of nuclear [N] and cytosol [C] fractions reveals that overexpression of INPP5F inhibits STAT3 nuclear translocation while INPP5F knockdown enhances the nuclear translocation of STAT3. (C) Control and INPP5F overexpressing GSC923 cells were treated with IL-6 (50 ng/ml) for 5 and 10 min, and immunoblot was performed with anti-p-STAT3 (Y705). Immunoblot analysis shows that INPP5F inhibits IL-6 induced phosphorylation of STAT3. (D) Confocal microscopy analysis verified that overexpression of INPP5F decreases STAT3 phosphorylation and nuclear translocation compared to control.
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- Figure 2 Pref-1 induces nucleocytoplasmic translocation of FOXO1 and PDX1 in PANC1 cells. ( a ) Immunoblot analyses and their quantification of ERK1/2, phosphorylated FOXO1, FOXO1, and PDX1 at day 2 after transfection with either pSPORT6-hDLK1 as Pref-1 or pCMV as control. Cells were treated with 25 muM PD98059 or 20 muM LY294002. n = 3, * P < 0.05 vs pCMV, # P < 0.05 between groups (means +- SE). ( b ) Protein levels of FOXO1 and PDX1 in the cytoplasmic and nuclear fractions at the indicated times after Pref-1 transfection, and their quantification. n = 3, ** P < 0.01 vs day 0 (means +- SE). ( c ) Immunostaining of PDX1 and FOXO1 at day 6 after transfection with either pSPORT6-hDLK1 or pCMV. Scale bar, 5 mum.
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- FIGURE 8: PUGNAc-induced stabilization of O -GlcNAcylation promotes nuclear exportation and degradation of ZO-2. Sparse cultures of MDCK cells were treated or not for 16 h with 100 muM PUGNAc or 100 muM PUGNAc plus 30 mM MG132. (A) Western blot detection of O -GlcNAc in MDCK cell extracts treated or not with PUGNAc (left). PUGNAc induces the disappearance of ZO-2 from the nuclei and cellular borders. Monolayers were fixed and processed for immunofluorescence with an antibody against ZO-2. The quantitative analysis of ZO-2 fluorescence signal intensity in the nucleus and cytoplasm was made with ImageJ particle analysis (bottom right). Results from three independent experiments. * p < 0.05 and ** p < 0.01 as assessed by two-way ANOVA followed by Bonferroni's post hoc test. (B) Treatment with PUGNAc decreases nuclear ZO-2. Left, Western blot against ZO-2 of nuclear (N), cytoplasmic (C), and plasma membrane (PM) fractions derived from cells treated or not with PUGNAc or PUGNAc plus MG132. Antibodies against beta 1 -Na + -K + -ATPase, actin, and lamin B1 were used as positive controls of PM, C, and N fractions, respectively. Right, densitometric analysis of the Western blot. Results from three independent experiments. * p < 0.05, ** p < 0.01, and **** p < 0.0001 as assessed by two-way ANOVA followed by Bonferroni's post hoc test. (C) Treatment with PUGNAc ablates the expression of O -GlcNAcylated ZO-2. The Duolink in situ immunoassay was done with an antibody against ZO-
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- Figure 3 Effects of melittin on PgLPS-induced activation of NF-kappaB signaling pathway, Akt, and ERK1/2. Representative Western blot images show the effects of PgLPS and melittin on the activation of cytosolic NF-kappaB inhibitor (IkappaB), nuclear NF-kappaB ( A ); Akt, and ERK1/2 ( B ). The bar graphs show quantitative signal intensities of the proteins after normalization with glyceraldehyde 3-phosphate dehydrogenase (GAPDH), Lamin B 1 , Akt, and ERK 1/2, respectively. -: untreated, +: treated. * p < 0.05 compared to the untreated group. + p < 0.05 compared to the PgLPS group. ( C ) Representative immunofluorescence images show the effects of PgLPS and melittin on the activation of NF-kappaB (labeled with Alexa Fluor 647, red) in HaCaT cells. The nuclei were labeled with Hoechst 33342 (blue). beta-actin was labeled with Alexa Fluor 488 (green). PgLPS: 0.1 mug/mL of P. gingivalis lipopolysaccharides, Melittin: 1 mug/mL of melittin.
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- FIGURE 4: ZO-2 is sequestered in the cytoplasm by 14-3-3 in the LC condition. (A) cZO-2 and hZO-2 sequences, respectively, have 25 and 22 putative 14-3-3 binding sites, 11 (cZO-2) and 10 (hZO-2) of which are located at the U2 segment. Scheme showing the molecular organization of ZO-2. PDZ, PDZ domain; SH3, Src homologous 3 domain; GK, guanylate kinase domain; Acidic, acidic region; PR, proline-rich segment; ABR, actin binding region; U, unique region; TEL, PDZ binding motif; NLS, nuclear localization signal; NES, nuclear exportation signal; vertical lines, putative 14-3-3 binding sites; blue letters and numbers, putative 14-3-3 binding sites located within an SR motif. (B) The inhibition of 14-3-3 with BV02 induces the appearance of ZO-1 and ZO-2 at the cell borders of cells cultured in LC. MDCK cells were plated at confluent density in NC media and after 1 h were washed 5x with PBS without Ca 2+ and transferred to LC media with 50 muM BV02 or vehicle only (DMSO 0.25%, control). After 20 h the monolayers were fixed and processed for immunofluorescence with antibodies against ZO-1, ZO-2, claudin-1, occludin, and cingulin. Top panel, representative images from three independent experiments. Nuclei were stained in blue with DAPI. Bar, 25 mum. Bottom panel, quantification of ZO-1 and ZO-2 fluorescence staining at the cell borders. Statistical analysis done with Student's t test, **** p < 0.0001. Results obtained from six optical fields in each experimental condition. (C) The cell
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- FIGURE 12: ZO-2 colocalizes with 14-3-3 in the nucleus, and residue S261 of cZO-2 is crucial for the nuclear importation the protein. (A) Left panel, percentage of cells with nuclear HA-ZO-2 as a function of time after transfection, determined by immunofluorescence using an anti HA antibody. Monolayers cultured in NC media were fixed at the indicated times. Time 0 corresponds to 6 h after transfection. Experiments were done with cells transfected with HA-cZO-2 WT, HA-cZO-2 S261A, and HA-cZO-2 T248A. Results are from three independent experiments. In each experiment, 100 transfected cells were evaluated per experimental point. Statistical analysis done with two way ANOVA followed by Bonferroni's multiple comparison test. cZO-2 S261A and HA-cZO-2 T248A were compared against HA-cZO-2 WT; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. (B) Western blot against HA-ZO-2 present in nuclear, cytoplasmic, and plasma membrane fractions of cells at times 0 and 24 h of transfection with HA-cZO-2 WT and HA-cZO-2 S261A. Antibodies against lamin B1, Na + ,K + -ATPase beta1 subunit, and GAPDH were employed as markers of nuclear, plasma membrane, and cytoplasmic fractions, respectively. Right panel, overexposed Western blot. (C) The reduced cellular content of HA-cZO-2 mutants T248A and S261A in comparison to WT HA-cZO-2 in LC cultured cells, recovers after proteasome inhibition with MG132. Western blot of cellular lysates derived from monolayers transfected with WT HA-cZO-2 or the m
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- Fig 2 The EGFR localization in nuclear and cytoplasmic fractions after treatment of A431 cells with sHB-EGF. Non-nuclear and nuclear cellular extracts were subjected to western-blot analysis after sHB-EGF treatment in the absence or presence of EGFR tyrosine kinase inhibitor AG1478. Down-regulation of EGFR activity by kinase inhibitor AG1478 suppressed the EGFR nuclear localization in A431 cells under sHB-EGF treatment.