PB9611

antibody from Boster Biological Technology

Targeting: LMNB1

Provider product page for PB9611

Western blot

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [49]
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Validations
Western blot [1]

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Product number: PB9611 - Provider product page
Provider: Boster Biological Technology
Product name: Anti-Lamin B1/LMNB1 Antibody Picoband™
Antibody type: Polyclonal
Description: Polyclonal antibody for Lamin B1/LMNB1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, IHC-F, ICC, ICC/IF, FCM. Reactive species: Human. Lamin B1/LMNB1 information: Molecular Weight: 66408 MW; Subcellular Localization: Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Vial size: 100μg/vial
Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Handling: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

LMNB1 protein structure - PB9611 shown in red.

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Submitted by: Boster Biological Technology (provider)
Main image
Experimental details: Western blot analysis of Lamin B1 using anti-Lamin B1 antibody (PB9611). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: U87 Whole Cell Lysate, Lane 2: PC-12 Whole Cell Lysate, Lane 3: NIH3T3 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B1 antigen affinity purified polyclonal antibody (Catalog # PB9611) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin B1 at approximately 67KD. The expected band size for Lamin B1 is at 67KD.
Additional image