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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- STJ99312 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-LMNB1 antibody [5G8-D3-H7] (STJ99312)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Lamin-B1 is suitable for use in Western Blot research applications.
- Reactivity
- Human, Mouse, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of Lamin B1 in COS7, Molt-4, A549 and 3T3 cell lysates using Lamin B1 mouse mAb (dilution 1:500).Predicted band size:68kDa.Observed band size:68kDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of Hela cells fixed with 4% Paraformaldehyde and using anti-Lamin B1 mouse mAb (dilution 1:100).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
