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Learn66095-1-IG
antibody from Invitrogen Antibodies
Targeting: LMNB1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 66095-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Lamin B1 Monoclonal Antibody (3C10G12)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: EYEYKLAQA LHEMREQHDA QVRLYKEELE QTYHAKLENA RLSSEMNTST VNSAREELME SRMRIESLSS QLSNLQKESR ACLERIQELE DLLAKEKDNS RRMLTDKERE MAEIRDQMQQ QLNDYEQLLD VKLALDMEIS AYRKLLQGEE ERLKLSPSPS SRVTVSRASS SRSVRTTRGK RKRVDVEESE ASSSVSISHS ASATGNVCIE EIDVDGKFIR LKNTSEQDQP MGGWEMIRKI GDTSVSYKYT SRYVLKAGQT VTIWAANAGV TASPPTDLIW KNQNSWGTGE DVKVILKNSQ GEEVAQRSTV FKTTIPEEEE EEEEAAGVVV EEELFHQQGT PRASNRSCAI M (237-587 aa encoded by BC012295)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3C10G12
- Vial size
- 150 µL
- Concentration
- 1.07 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot on multiple cells/tissues with anti-LMNB1 (66095-1-IG) at dilution 1:20000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot on multiple cells/tissues with anti-LMNB1 (66095-1-IG) at dilution 1:20000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Hela cell with anti-LMNB1 (66095-1-IG) at various dilutions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of Lamin B1 antibody (66095-1-IG, 1:300,000) with si-Control and si-Lamin B1 transfected HepG2 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 66095-1-IG (Lamin B1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 66095-1-IG ( Lamin B1 antibody) at dilution of 1:1000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 66095-1-IG ( Lamin B1 antibody) at dilution of 1:1000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug Lamin B1 antibody (66095-1-IG, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IGG with dilution 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Lamin B1 (IP:66095-1-IG, 4ug; Detection:66095-1-IG 1:20000) with HeLa cells lysate 3560ug.
