Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- Ab112658 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Lamin B1 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human Lamin B1 Antibody, Monoclonal (C2), could be used for WB, IHC and so on.Application: WB: 0.1ng/ml-100ng/mLIHC: 5-20_g/mLProtein FunctionLamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.Post-translationalB-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Porcine, Rabbit
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 52-233
- Antibody clone number
- C2
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Lamin B1 Mouse mAb (Ab112658) - Western Blot All lanes: Lamin B1 Mouse mAb (Ab112658) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 66 kDa Observed band size: 68 kDa Exposure time: 30 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Lamin B1 Mouse mAb (Ab112658) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658) Second Ab: 2µg/mLHRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody .
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Lamin B1 Mouse mAb (Ab112658) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Lamin B1 Mouse mAb (Ab112658) - IHC DAB staining on IHC Samples: Human Ovarian cancer Tissue Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Lamin B1 Mouse mAb (Ab112658) - IHC DAB staining on IHC Samples: Human Thyroid cancer Tissue Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Lamin B1 Mouse mAb (Ab112658) - IHC DAB staining on IHC Samples: Human amy.gdalitis Tissue Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Lamin B1 Mouse mAb (Ab112658) - IHC DAB staining on IHC Samples: Human Lung cancer Tissue Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.