Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- Ab112659 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Lamin B1 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human Lamin B1 Antibody, Recombinant, could be used for WB, IHC and so on.Application:WB: 1/2000 - 1/10000 IHC: 1/50 - 1/200Protein FunctionLamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.Post-translationalB-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 527-540
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,3 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Lamin B1 Antibody (Ab112659) - Western Blot All lanes: Recombinant Lamin B1 Antibody (Ab112659) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 66 kDa Observed band size: 66, 22 kDa Exposure time: 0.9 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Lamin B1 Antibody (Ab112659) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Lamin B1 Antibody (Ab112659). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.