Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-32474 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Lamin B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Inactivation of XPF Sensitizes Cancer Cells to Gemcitabine.
COPI-Mediated Nuclear Translocation of EGFRvIII Promotes STAT3 Phosphorylation and PKM2 Nuclear Localization.
George JW, Bessho M, Bessho T
Journal of nucleic acids 2019;2019:6357609
Journal of nucleic acids 2019;2019:6357609
COPI-Mediated Nuclear Translocation of EGFRvIII Promotes STAT3 Phosphorylation and PKM2 Nuclear Localization.
Zhang M, Sun H, Deng Y, Su M, Wei S, Wang P, Yu L, Liu J, Guo J, Wang X, Han X, He Q, Shen L
International journal of biological sciences 2019;15(1):114-126
International journal of biological sciences 2019;15(1):114-126
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HepG2 Cell Lysate using anti-Lamin B Polyclonal Antibody (Product # PA5-32474). The recommened dilution for this antibody in western blot applications is 1:25.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), Hep G2 (Lane 3), HEK293T (Lane 4), U-2 OS (Lane 5) and Jurkat (Lane 6). The blot was probed with Anti-Lamin B1 Polyclonal Antibody (Product # PA5-32474, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 65 kDa band corresponding to Lamin B1 was observed across the cell lines tested.