Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [4]
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Validation data
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- Product number
- NBP2-22217AF647 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal LAMP-2/CD107b Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [Alexa Fluor® 647] [NBP2-22217AF647] - An intracellular stain was performed on HeLa cells with LAMP-2/CD107b (H4B4) NBP2-22217AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [Alexa Fluor® 647] [NBP2-22217AF647] - An intracellular stain was performed on SK-MEL-28 cells with LAMP-2/CD107b (H4B4) NBP2-22217AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [Alexa Fluor® 647] [NBP2-22217AF647] - An intracellular stain was performed on Jurkat cells with LAMP-2/CD107b [H4B4] Antibody NBP2-22217AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [Alexa Fluor® 647] [NBP2-22217AF647] - An intracellular stain was performed on U937 cells with LAMP-2/CD107b [H4B4] Antibody NBP2-22217AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.