IC6228G

antibody from Novus Biologicals

Targeting: LAMP2 CD107b

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC6228G

Provider

Novus Biologicals

Product name

Mouse Monoclonal LAMP-2/CD107b Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Conjugated LAMP2/CD107b antibodies are ideal for immunocytochemistry colocalization studies in lysosomes. The unconjugated antibody detects human LAMP2/CD107b in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LAMP1 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of LAMP2/CD107b in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human LAMP2/CD107b Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC6228G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.