Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14-1078-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD107b (LAMP-2) Monoclonal Antibody (eBioH4B4 (H4B4)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioH4B4 monoclonal antibody reacts with human CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2). CD107b is a highly glycosylated, type I transmembrane protein of approximately 105 kDa. It is expressed intracellularly in lysosomal/endosomal membranes in nearly all cells. It is also expressed on the surface of degranulating T cells (to a lesser extent than CD107a) and activated platelets as well as some cancer cells. In humans, mutations in CD107b results in a lysosomal glycogen storage disorder, known as Danon disease. Applications Reported: Purified anti-human CD107b (LAMP-2) has been reported for use in flow cytometric analysis. It has also been reported for use in surface staining in a flow cytometric based degranulation assay. (Fluorochrome conjugated eBioH4B4 (H4B4) is recommended for use in flow cytometry.). Applications Tested: This eBioH4B4 (H4B4) antibody has been tested by intracellular staining and flow cytometric analysis of Jurkat cell line. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This antibody has also been tested by immunoblotting at 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBioH4B4 (H4B4)
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Improved Autophagic Flux in Escapers from Doxorubicin-Induced Senescence/Polyploidy of Breast Cancer Cells.
Lysosomal membrane permeabilization is involved in oxidative stress-induced apoptotic cell death in LAMP2-deficient iPSCs-derived cerebral cortical neurons.
The Tumor Antigen NY-ESO-1 Mediates Direct Recognition of Melanoma Cells by CD4+ T Cells after Intercellular Antigen Transfer.
Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans.
Detection of T-cell degranulation: CD107a and b.
LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells.
Role of LAMP-2 in lysosome biogenesis and autophagy.
Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease).
Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins carrying polylactosaminoglycan.
Bojko A, Staniak K, Czarnecka-Herok J, Sunderland P, Dudkowska M, Śliwińska MA, Salmina K, Sikora E
International journal of molecular sciences 2020 Aug 24;21(17)
International journal of molecular sciences 2020 Aug 24;21(17)
Lysosomal membrane permeabilization is involved in oxidative stress-induced apoptotic cell death in LAMP2-deficient iPSCs-derived cerebral cortical neurons.
Law CY, Siu CW, Fan K, Lai WH, Au KW, Lau YM, Wong LY, Ho JCY, Lee YK, Tse HF, Ng KM
Biochemistry and biophysics reports 2016 Mar;5:335-345
Biochemistry and biophysics reports 2016 Mar;5:335-345
The Tumor Antigen NY-ESO-1 Mediates Direct Recognition of Melanoma Cells by CD4+ T Cells after Intercellular Antigen Transfer.
Fonteneau JF, Brilot F, Münz C, Gannagé M
Journal of immunology (Baltimore, Md. : 1950) 2016 Jan 1;196(1):64-71
Journal of immunology (Baltimore, Md. : 1950) 2016 Jan 1;196(1):64-71
Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans.
McArthur MA, Sztein MB
Clinical immunology (Orlando, Fla.) 2013 Feb;146(2):140-52
Clinical immunology (Orlando, Fla.) 2013 Feb;146(2):140-52
Detection of T-cell degranulation: CD107a and b.
Betts MR, Koup RA
Methods in cell biology 2004;75:497-512
Methods in cell biology 2004;75:497-512
LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells.
Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2004 Sep;61(1):62-8
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2004 Sep;61(1):62-8
Role of LAMP-2 in lysosome biogenesis and autophagy.
Eskelinen EL, Illert AL, Tanaka Y, Schwarzmann G, Blanz J, Von Figura K, Saftig P
Molecular biology of the cell 2002 Sep;13(9):3355-68
Molecular biology of the cell 2002 Sep;13(9):3355-68
Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease).
Nishino I, Fu J, Tanji K, Yamada T, Shimojo S, Koori T, Mora M, Riggs JE, Oh SJ, Koga Y, Sue CM, Yamamoto A, Murakami N, Shanske S, Byrne E, Bonilla E, Nonaka I, DiMauro S, Hirano M
Nature 2000 Aug 24;406(6798):906-10
Nature 2000 Aug 24;406(6798):906-10
Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins carrying polylactosaminoglycan.
Carlsson SR, Roth J, Piller F, Fukuda M
The Journal of biological chemistry 1988 Dec 15;263(35):18911-9
The Journal of biological chemistry 1988 Dec 15;263(35):18911-9
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells (P) and Jurkat cell line (J) lysates were immunoblotted with 1 µg/mL of Anti-Human CD107b (LAMP-2) Purified and revealed with Anti-Mouse HRP.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Autophagic flux resumption in descendants of polyploid/senescent MDA-MB-231 and MCF-7 cells. Cells were treated with 100 nM doxorubicin for one day, then cultured in a fresh medium and analyzed on subsequent days. ( a , c ) Representative western blots showing autophagy protein levels of p-ULK1 (S757), ULK1, LC3B, LAMP-2 and SQSTM1/p62 in MDA-MB-231 cells ( a ) and MCF-7 cells ( c ). ( b , d ) Quantitative analysis of autophagic index based on LC3B protein levels in untreated and bafilomycin A- or chloroquine-treated MDA-MB-231 cells ( b ) and MCF-7 cells ( d ) with representative western blots showing LC3B protein levels. Bars: mean value, error bars: SEM, n = 4. Statistical significance (in relation to control): $ p < 0.051, * p < 0.05, ** p < 0.01. ( e ) Transient accumulation of autophagic vesicles. TEM images show typical MDA-MB-231cells on the subsequent days following treatment (upper panel) and their magnified parts with autophagic vesicles (red arrows) and lipofuscin particles (red asterisks).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 LAMP2-deficient neurons showed increase levels of cytosolic cathepsin L. (A) Western blot analysis revealed the elevated level of active cathepsin L in the LAMP2-deficient iPSCs-derived cortical neurons under non-stressed condition. (B) Western blot analysis showed that oxidative stress increased the abundance of active cathepsin L in the cytosols. N =3; *: P