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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- MA5-17861 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-LAMP2 Monoclonal Antibody (M3/84)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17861 targets LAMP-2 in CT, FACS, IHC (F), and IP applications and shows reactivity with Mouse samples. The MA5-17861 immunogen is immunoadsorbent purified mouse macrophage glycoprotein fraction.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- M3/84
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of RAW 264.7 (Lane 1) and NIH/3T3 (Lane 2). The blots were probed with Anti-LAMP2 Monoclonal Antibody (Product # MA5-17861, 1:1000 dilution) and detected by chemiluminescence using Sheep anti-Rat IgG Secondary Antibody, HRP conjugate (Product # PA1-28642, 0.2 µg/ml, 1:5000 dilution). A 110 kDa band corresponding to glycosylated form of LAMP2 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Thioglycolate-elicited C57BL/6 mouse peritoneal macrophages were stained using a LAMP-2 monoclonal antibody (Product # MA5-17861) (filled histogram) or rat IgG1 isotype control (open histogram).
