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- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [4]
- Immunoprecipitation [2]
- Immunohistochemistry [3]
- Other assay [2]
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- Product number
- PA5-118026 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LAMP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of LAMP2 in A431 cells. Samples were treated with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, incubated with polyclonal antibody (Product # PA5-118026) with a dilution of 1:1,000 (4°C overnight), followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of LAMP2 in A431 cells. Samples were treated with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, incubated with polyclonal antibody (Product # PA5-118026) with a dilution of 1:1,000 (4°C overnight), followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of LAMP2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with LAMP2 Polyclonal Antibody (Product # PA5-118026, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of LAMP2 in A431 cells. Samples were treated with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, incubated with polyclonal antibody (Product # PA5-118026) with a dilution of 1:1,000 (4°C overnight), followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LAMP2 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate, Lane B: 0.5 mg 293T Whole Cell Lysate 2 µL with LAMP2 Polyclonal Antibody (Product # PA5-118026) and 15 µL of 50 % Protein G agarose. Primary antibody: LAMP2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 120 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of LAMP2 in Lane A: 0.5 mg HepG2 Whole Cell lysate, Lane B: 0.5 mg 293T Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with polyclonal antibody (Product # PA5-118026) with a dilution of 1:100 , followed by Dylight 800-labeled antibody to rabbit IgG (H+L) using a dilution of 1:5,000. Assay was performed under reducing conditions. Predicted band size: 45 kDa, Observed band size: 120 kDa .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) of LAMP2 in human liver. Samples were incubated with polyclonal antibody (Product # PA5-118026) with a dilution of 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) of LAMP2 in human placenta. Samples were incubated with polyclonal antibody (Product # PA5-118026) with a dilution of 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human LAMP2 in human placenta with LAMP2 Polyclonal Antibody (Product # PA5-118026, 1:5,000, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LAMP2 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate, Lane B: 0.5 mg 293T Whole Cell Lysate 2 µL with LAMP2 Polyclonal Antibody (Product # PA5-118026) and 15 µL of 50 % Protein G agarose. Primary antibody: LAMP2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 120 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of LAMP2 in Lane A: 0.5 mg HepG2 Whole Cell lysate, Lane B: 0.5 mg 293T Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with polyclonal antibody (Product # PA5-118026) with a dilution of 1:100 , followed by Dylight 800-labeled antibody to rabbit IgG (H+L) using a dilution of 1:5,000. Assay was performed under reducing conditions. Predicted band size: 45 kDa, Observed band size: 120 kDa .
