Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-B6889 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ c-Met Antibody (clone 4AT44) LS-B6889
- Antibody type
- Monoclonal
- Description
- Protein G purified
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4AT44
- Storage
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of lysates from HeLa, HepG2, COS-7 cell line (from left to right), using MET/HGFR Antibody(4AT44). 4AT44 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of endogenous Met in HepG2 cell line. 10 ug/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent different anti-Met monoclonal antibodies. Lane 6 represents auto-phosphorylated-Met in HepG2 cell line detected by anti-phospho-Met antibody.