Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-17770 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-c-Met (Tyr1234, Tyr1235) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-Phospho-c-Met (Tyr1234, Tyr1235) Polyclonal Antibody (Product # PA5-17770) and a 170 kDa band corresponding to Phospho-Hepatocyte growth factor receptor (Tyr1234, Tyr1235) was observed in A-431 cell line treated with EGF. Additional uncharacterized bands (*) were also observed below 60 kDa. Membrane enriched extracts (30 µg lysate) of A-431 (Lane 1) and A-431 treated with EGF (200 ng/mL for 10 min) (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).