25869-1-AP

antibody from Proteintech Group

Targeting: MET DFNB97, HGFR, RCCP2

Provider product page for 25869-1-AP

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [46]
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Validations
Western blot [1]
Immunoprecipitation [1]
Immunohistochemistry [2]

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Product number: 25869-1-AP - Provider product page
Provider: Proteintech Group
Product name: c-Met (Cytoplasmic) antibody
Antibody type: Polyclonal
Description: KD/KO validated c-Met (Cytoplasmic) antibody (Cat. #25869-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, canine, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat, Canine
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MET protein structure - 25869-1-AP shown in red.

Submitted references Construction of disulfidptosis-based immune response prediction model with artificial intelligence and validation of the pivotal grouping oncogene c-MET in regulating T cell exhaustion.

Li P, Wang S, Wan H, Huang Y, Yin K, Sun K, Jin H, Wang Z
Frontiers in immunology 2024;15:1258475

Inhibition of the AURKA/YAP1 axis is a promising therapeutic option for overcoming cetuximab resistance in colorectal cancer stem cells.

Rio-Vilariño A, Cenigaonandia-Campillo A, García-Bautista A, Mateos-Gómez PA, Schlaepfer MI, Del Puerto-Nevado L, Aguilera O, García-García L, Galeano C, de Miguel I, Serrano-López J, Baños N, Fernández-Aceñero MJ, Lacal JC, Medico E, García-Foncillas J, Cebrián A
British journal of cancer 2024 May;130(8):1402-1413

Parathyroid hormone-related protein as a potential prostate cancer biomarker: Promoting prostate cancer progression through upregulation of c-Met expression.

Zhao Y, Fu ZY, Feng HY, Peng YH, Yin ZX, Cao JY, Pei CS
Biomolecules & biomedicine 2024 Mar 11;24(2):374-386

MET exon 14 skipping mutation drives cancer progression and recurrence via activation of SMAD2 signalling.

Liang Q, Hu Y, Yuan Q, Yu M, Wang H, Zhao B
British journal of cancer 2024 Feb;130(3):380-393

Mesothelin promotes brain metastasis of non-small cell lung cancer by activating MET.

Xia S, Duan W, Xu M, Li M, Tang M, Wei S, Lin M, Li E, Liu W, Wang Q
Journal of experimental & clinical cancer research : CR 2024 Apr 3;43(1):103

IGF2BP2 promotes ovarian cancer growth and metastasis by upregulating CKAP2L protein expression in an m(6) A-dependent manner.

Shi Y, Xiong X, Sun Y, Geng Z, Chen X, Cui X, Lv J, Ge L, Jia X, Xu J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Oct;37(10):e23183

Improved anti-tumor activity of fluorinated camptothecin derivatives 9-fluorocamptothecin and 7-ethyl-9-fluorocamptothecin on hepatocellular carcinoma by targeting topoisomerase I.

Zhang M, Zhu LZ, Yang CJ, Yan JX, Wang ZP, Bai YP, Peng LZ, Luo HB, Zhang ZJ, Li L, Xu CR, Liu YQ
Bioorganic chemistry 2023 Oct;139:106652

RFC2 promotes aerobic glycolysis and progression of colorectal cancer.

Lou F, Zhang M
BMC gastroenterology 2023 Oct 11;23(1):353

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy.

Wu JC, Wang XJ, Zhu JH, Huang XY, Liu M, Qiao Z, Zhang Y, Sun Y, Wang ZY, Zhan P, Zhang T, Hu HL, Liu H, Tang W, Yi F
Acta pharmacologica Sinica 2023 Jun;44(6):1206-1216

Clinicopathological features and immunophenotype of Silva pattern system in endocervical adenocarcinoma.

Zeng C, Wu JK, Lu X
International journal of experimental pathology 2023 Jun;104(3):140-150

Cytostatic Activity of Sanguinarine and a Cyanide Derivative in Human Erythroleukemia Cells Is Mediated by Suppression of c-MET/MAPK Signaling.

Xu X, Deng L, Tang Y, Li J, Zhong T, Hao X, Fan Y, Mu S
International journal of molecular sciences 2023 Apr 30;24(9)

Identification and validation of autophagy-related gene expression for predicting prognosis in patients with idiopathic pulmonary fibrosis.

Huang G, Xu X, Ju C, Zhong N, He J, Tang XX
Frontiers in immunology 2022;13:997138

Time-extended exposure of gastric epithelial cells to secretome of Helicobacter pylori-activated fibroblasts induces reprogramming of gastric epithelium towards pre-cancerogenic and pro-invasive phenotype.

Krzysiek-Maczka G, Targosz A, Wrobel T, Paw M, Szczyrk U, Opila J, Strzalka M, Wierdak M, Major P, Brzozowski T, Czyz J, Ptak-Belowska A
American journal of cancer research 2022;12(3):1337-1371

A four oxidative stress gene prognostic model and integrated immunity-analysis in pancreatic adenocarcinoma.

Wang H, Tian RF, Liang X, Fan J, Duan ZC, Fan XY, Zhang JJ, Yao DS, Chen ZN, Li L
Frontiers in oncology 2022;12:1015042

Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop.

Shen Y, Zhu Q, Xiao M, Yin L, Feng W, Feng J, He J, Li P, Chen X, Ding W, Zhong J, Zeng Z, Xie Z, Liu J, Zu X
Cell death & disease 2022 Aug 30;13(8):749

ORP5 promotes tumor metastasis via stabilizing c-Met in renal cell carcinoma.

Song L, Zhang L, Zhou Y, Shao X, Xu Y, Pei D, Wang Q
Cell death discovery 2022 Apr 21;8(1):219

Combined Treatment of Cinobufotalin and Gefitinib Exhibits Potent Efficacy against Lung Cancer.

Han Y, Ma R, Cao G, Liu H, He L, Tang L, Li H, Luo Q
Evidence-based complementary and alternative medicine : eCAM 2021;2021:6612365

Long noncoding RNA SH3PXD2A-AS1 promotes colorectal cancer progression by regulating p53-mediated gene transcription.

Hou P, Lin T, Meng S, Shi M, Chen F, Jiang T, Li Z, Li M, Chu S, Zheng J, Bai J
International journal of biological sciences 2021;17(8):1979-1994

Development and Validation of a Nomogram based on cell growth-related Biomarkers for Oral Squamous Cell Carcinoma.

Shuai Y, Duan Y, Zhou M, Yue K, Liu D, Fang Y, Wang Y, Wu Y, Zhang Z, Wang X
Journal of Cancer 2021;12(17):5153-5163

Hepatocyte Growth Factor Overexpression Slows the Progression of 4NQO-Induced Oral Tumorigenesis.

He X, Chen S, Tang Y, Zhao X, Yan L, Wu L, Wu Z, Liu W, Chen X, Wang X
Frontiers in oncology 2021;11:756479

Development of bispecific anti-c-Met/PD-1 diabodies for the treatment of solid tumors and the effect of c-Met binding affinity on efficacy.

Yuan Q, Liang Q, Sun Z, Yuan X, Hou W, Wang Y, Wang H, Yu M
Oncoimmunology 2021;10(1):1914954

Combinational blockade of MET and PD-L1 improves pancreatic cancer immunotherapeutic efficacy.

Li E, Huang X, Zhang G, Liang T
Journal of experimental & clinical cancer research : CR 2021 Sep 3;40(1):279

CXCR4 promotes gefitinib resistance of Huh7 cells by activating the c-Met signaling pathway.

Zhao D, Yang Z, Chen C, Zhang Z, Yu Y, Li Z
FEBS open bio 2021 Nov;11(11):3115-3125

Effects of Cynaroside on Cell Proliferation, Apoptosis, Migration and Invasion though the MET/AKT/mTOR Axis in Gastric Cancer.

Ji J, Wang Z, Sun W, Li Z, Cai H, Zhao E, Cui H
International journal of molecular sciences 2021 Nov 9;22(22)

miRNA in food simultaneously controls animal viral disease and human tumorigenesis.

Cui Y, Wan H, Zhang X
Molecular therapy. Nucleic acids 2021 Mar 5;23:995-1006

Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors.

Yuan X, Sun Z, Yuan Q, Hou W, Liang Q, Wang Y, Mo W, Wang H, Yu M
Investigational new drugs 2021 Feb;39(1):34-51

Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.

Dong X, Luo Y, Lu S, Ma H, Zhang W, Zhu Y, Sun G, Sun X
Clinical and translational medicine 2021 Feb;11(2):e296

Trichostatin A downregulates bromodomain and extra-terminal proteins to suppress osimertinib resistant non-small cell lung carcinoma.

Meng Y, Qian X, Zhao L, Li N, Wu S, Chen B, Sun T, Wang X
Cancer cell international 2021 Apr 15;21(1):216

Tivantinib Decreases Hepatocyte Growth Factor-Induced BCRP Expression in Hepatocellular Carcinoma HepG2 Cells.

Kobayashi K, Higai K, Mukozu T, Matsui D, Amanuma M, Yoshimine N, Ogino Y, Matsui T, Wakui N, Shinohara M, Momiyama K, Daido Y, Nagai H, Igarashi Y
Biological & pharmaceutical bulletin 2020;43(9):1421-1425

Adipose-Derived Stem Cells Promote Proliferation and Invasion in Cervical Cancer by Targeting the HGF/c-MET Pathway.

Zhai Y, Wu W, Xi X, Yu R
Cancer management and research 2020;12:11823-11832

The long noncoding RNA FTX promotes a malignant phenotype in bone marrow mesenchymal stem cells via the miR-186/c-Met axis.

Liu L, Li X, Shi Y, Chen H
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 Nov;131:110666

Decorin promotes apoptosis and autophagy via suppressing c-Met in HTR-8 trophoblasts.

Wang Y, Zhang H, Zhang Y, Li X, Hu X, Wang X
Reproduction (Cambridge, England) 2020 May;159(6):669-677

Human-like collagen promotes the healing of acetic acid-induced gastric ulcers in rats by regulating NOS and growth factors.

Xing M, Fu R, Liu Y, Wang P, Ma P, Zhu C, Fan D
Food & function 2020 May 1;11(5):4123-4137

Increased expression of hematological and neurological expressed 1 (HN1) is associated with a poor prognosis of hepatocellular carcinoma and its knockdown inhibits cell growth and migration partly by down-regulation of c-Met.

Chen JJ, Sun X, Mao QQ, Jiang XY, Zhao XG, Xu WJ, Zhong L
The Kaohsiung journal of medical sciences 2020 Mar;36(3):196-205

Pulp stem cells with hepatocyte growth factor overexpression exhibit dual effects in rheumatoid arthritis.

Dong X, Kong F, Liu C, Dai S, Zhang Y, Xiao F, Zhang H, Wu CT, Wang H
Stem cell research & therapy 2020 Jun 10;11(1):229

CAPN1 promotes malignant behavior and erlotinib resistance mediated by phosphorylation of c-Met and PIK3R2 via degrading PTPN1 in lung adenocarcinoma.

Chen Y, Tang J, Lu T, Liu F
Thoracic cancer 2020 Jul;11(7):1848-1860

CCND1, NOP14 and DNMT3B are involved in miR-502-5p-mediated inhibition of cell migration and proliferation in bladder cancer.

Ying Y, Li J, Xie H, Yan H, Jin K, He L, Ma X, Wu J, Xu X, Fang J, Wang X, Zheng X, Liu B, Xie L
Cell proliferation 2020 Feb;53(2):e12751

SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis.

Liu R, Shuai Y, Luo J, Zhang Z
Frontiers in oncology 2019;9:1035

Rosiglitazone Suppresses Calcium Oxalate Crystal Binding and Oxalate-Induced Oxidative Stress in Renal Epithelial Cells by Promoting PPAR-γ Activation and Subsequent Regulation of TGF-β1 and HGF Expression.

Liu YD, Yu SL, Wang R, Liu JN, Jin YS, Li YF, An RH
Oxidative medicine and cellular longevity 2019;2019:4826525

A novel tetravalent bispecific antibody targeting programmed death 1 and tyrosine-protein kinase Met for treatment of gastric cancer.

Hou W, Yuan Q, Yuan X, Wang Y, Mo W, Wang H, Yu M
Investigational new drugs 2019 Oct;37(5):876-889

The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.

Huang X, Gan G, Wang X, Xu T, Xie W
Autophagy 2019 Jul;15(7):1258-1279

Dual regulatory role of CCNA2 in modulating CDK6 and MET-mediated cell-cycle pathway and EMT progression is blocked by miR-381-3p in bladder cancer.

Li J, Ying Y, Xie H, Jin K, Yan H, Wang S, Xu M, Xu X, Wang X, Yang K, Zheng X, Xie L
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Jan;33(1):1374-1388

Clinicopathologic Features and Prognostic Factors in Alpha-Fetoprotein-Producing Colorectal Cancer: Analysis of 78 Cases.

Feng Y, Li Y, Dai W, Mo S, Li Q, Cai S
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;51(5):2052-2064

ISG15 promotes esophageal squamous cell carcinoma tumorigenesis via c-MET/Fyn/β-catenin signaling pathway.

Yuan H, Zhou W, Yang Y, Xue L, Liu L, Song Y
Experimental cell research 2018 Jun 1;367(1):47-55

Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer.

Cui Y, Yang X, Zhang X
Molecular therapy. Nucleic acids 2017 Dec 15;9:387-398

SOCS1 regulates hepatic regenerative response and provides prognostic makers for acute obstructive cholangitis.

Yu J, Zhang W, Qian H, Tang H, Lin W, Lu B
Scientific reports 2017 Aug 25;7(1):9482

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: A431 cells were subjected to SDS PAGE followed by western blot with 25869-1-AP( MET Antibody) at dilution of 1:300
Validation comment: WB
Appendix: 25869-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-MET (IP:25869-1-AP, 5ug; Detection:25869-1-AP 1:300) with HeLa cells lysate 1600ug.
Validation comment: IP
Appendix: 25869-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 25869-1-AP( MET Antibody) at dilution of 1:50 (under 10x lens)
Validation comment: IHC
Appendix: 25869-1-AP.pdf

: Show more

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 25869-1-AP( MET Antibody) at dilution of 1:50 (under 40x lens)
Validation comment: IHC
Appendix: 25869-1-AP.pdf

: Show more