AM1002A

antibody from Abcepta

Targeting: MET DFNB97, HGFR, RCCP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM1002A

Provider

Abcepta

Proper citation

Abgent Cat#AM1002a, RRID:AB_2143876

Product name

MET/HGFR Antibody

Antibody type

Monoclonal

Antigen

Other

Description

Purified Mouse Monoclonal Antibody (Mab)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

400 µl

Concentration

0.49 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Detection of endogenous Met in HepG2 cell line. 10 ug/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent Abgent ’s different anti-Met monoclonal antibodies that are Cat# AM1001-1005. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab (Cat# AM1000).

Primary Ab dilution

1:100~500

Immunocytochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Fluorescent confocal image of HepG2 cells stained with MET/HGFR antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1002a MET/HGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). ). Note the highly specific localization of the MET immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000105976). "

Primary Ab dilution

1:100

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

MET/HGFR Antibody (Cat. #AM1002a) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the MET/HGFR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:50~100