NBP2-44306

antibody from Novus Biologicals

Targeting: MET DFNB97, HGFR, RCCP2

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-44306

Provider

Novus Biologicals

Product name

Mouse Monoclonal HGFR/c-MET Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - Analysis of HGF R/c-Met (1G7NB) in human liver cancer.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Intracellular): HGF R/c-MET Antibody (1G7NB) [NBP2-44306] - An intracellular stain was performed on HeLa cells with NBP2-44306PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Intracellular): HGF R/c-MET Antibody (1G7NB) [NBP2-44306] - An intracellular stain was performed on HeLa cells with HGF R/c-MET [1G7NB] Antibody NBP2-44306AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - c-MET antibody was tested in HeLa cells (1 x 10^6 cells/ml). After fixation and permeabilization, cells were stained using the anti-c-MET antibody (clone 1G7NB) at a 1:1000 dilution. Signal was detected using a Goat anti-Mouse Dylight 488 secondary (blue peak). Shown with secondary control (orange peak). Data was acquired on BD FACSCalibur. Image using the Azide Free format of this antibody.