LS-C682403

antibody from LSBio

Targeting: MET DFNB97, HGFR, RCCP2

Western blot (Enhanced validation)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C682403

Provider

LSBio

Product name

c-Met Antibody (clone 5E9B7) LS-C682403

Antibody type

Monoclonal

Description

Purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5E9B7

Storage

Short term: store at 4°C. Long term: store at -20°C.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using MET mAb against HEK293 (1) and MET (AA: 743-932)-hIgGFc transfected HEK293 (2) cell lysate.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using MET mAb against human MET (AA: 743-932) recombinant protein. (Expected MW is 47 kDa)

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence analysis of Hela cells using MET mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence analysis of Hela cells using MET mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher