PA5-27235

antibody from Invitrogen Antibodies

Targeting: MET DFNB97, HGFR, RCCP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27235

Provider

Invitrogen Antibodies

Product name

c-Met Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: HeLa, HeLaS3.

Concentration

1.95 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MET using 30 µg of HeLa S3 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a MET polyclonal antibody (Product # PA5-27235) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using c-Met Polyclonal Antibody (Product # PA5-27235). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met Polyclonal Antibody (Product # PA5-27235) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of c-Met was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: c-Met Polyclonal Antibody (Product # PA5-27235) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.