Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN1504180 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Met Proto-Oncogene (Hepatocyte Growth Factor Receptor) (MET) antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Supernatant
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 'NL8
- Vial size
- 0.1 mL
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Western Blotting Analysis (Representative Lot Data): Untreated (Lane -) and ?-phosphatase treated (Lane +) RIPA lysate from A431 cells co-expressing Met/HGFR and HGF were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-Met (Tyr1234/1235), clone NL8 (1:10,000 dilution).Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.Arrow indicates phospho-Met (~140 kDa)