Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN1504566 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Met Proto-Oncogene (Hepatocyte Growth Factor Receptor) (MET) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunoaffinity Chromatography
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mL
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Immunoblot Analysis: Lysates from untreated (Lane 1) or EGF treated (Lanes 2 and 3) A431 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phospho-Met (Tyr1003) for 2 hours at room temperature. Lane 3 was pre-incubated with the blocking peptide immunogen corresponding to phospho-Met (Tyr1003). Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Met (Tyr1003).