Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX22828 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX22828, RRID:AB_372299
- Product name
- MECP2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Drosophila, Simian
- Host
- Rabbit
Submitted references Cocaine-mediated activation of microglia and microglial MeCP2 and BDNF production.
Cotto B, Li H, Tuma RF, Ward SJ, Langford D
Neurobiology of disease 2018 Sep;117:28-41
Neurobiology of disease 2018 Sep;117:28-41
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of HeLa cell lysate using GTX22828 MECP2 antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of BEAS-2B cell lysate using GTX22828 MECP2 antibody.Dilution : 1:1500
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of C6 cells (right) compared to a negative control without primary antibody (left) using GTX22828 MECP2 antibody.Dilution : 1:200Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of C2C12 cells (right) compared to a negative control without primary antibody (left) using GTX22828 MECP2 antibody.Green : Primary antibodyRed : Actin Blue : DAPIDilution : 1:200Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of rat brain tissue (right) compared to a negative control without primary antibody (left) using GTX22828 MECP2 antibody.Dilution : 1:1000Antigen retrieval : 10mM sodium citrate (pH 6.0)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of mouse brain tissue (right) compared to a negative control without primary antibody (left) using GTX22828 MECP2 antibody.Dilution : 1:500Antigen retrieval : 10mM sodium citrate (pH 6.0)