PA5-118563

antibody from Invitrogen Antibodies

Targeting: CALM3 PHKD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118563

Provider

Invitrogen Antibodies

Product name

Phospho-Calmodulin 1/2/3 (Thr79, Ser81) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-Calmodulin 1/2/3 (Thr79, Ser81) in HeLa cells using a Phospho-Calmodulin 1/2/3 (Thr79, Ser81) polyclonal antibody (Product # PA5-118563). Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Phospho-Calmodulin 1/2/3 (Thr79, Ser81) in HepG2 cells using a Phospho-Calmodulin 1/2/3 (Thr79, Ser81) polyclonal antibody (Product # PA5-118563). The samples were with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1:400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1:600 was used as secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Phospho-Calmodulin 1/2/3 (Thr79, Ser81) in HepG2 cells using a Phospho-Calmodulin 1/2/3 (Thr79, Ser81) polyclonal antibody (Product # PA5-118563). The samples were with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1:400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1:600 was used as secondary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-Calmodulin 1/2/3 (Thr79, Ser81) in human brain tissue using a Phospho-Calmodulin 1/2/3 (Thr79, Ser81) polyclonal antibody (Product # PA5-118563). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-Calmodulin 1/2/3 (Thr79, Ser81) in Human prostate using a Phospho-Calmodulin 1/2/3 (Thr79, Ser81) polyclonal antibody (Product # PA5-118563). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.