Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
Reference
Comment
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- Product number
- AB7818-200 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB7818-200, RRID:AB_2333163
- Product name
- Anti-Rab1
- Antibody type
- Polyclonal
- Description
- RAB2 belongs to the large RAB family of low molecular weight GTPases that are involved in intracellular membrane trafficking. It has been demonstrated that Rab2 is essential for the maturation of pre-Golgi intermediates. RAB2A and RAB2B are required for protein transport from the ER to the Golgi, RAB2A is lipid-anchored to the ER-Golgi intermediate compartment membrane while RAB2B is lipid anchored to the cytoplasmic side of the cell membrane. RAB2A has been shown to interact with GOLGA2.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 600 µg
- Concentration
- 3 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references AmotL2 disrupts apical–basal cell polarity and promotes tumour invasion
Mojallal M, Zheng Y, Hultin S, Audebert S, van Harn T, Johnsson P, Lenander C, Fritz N, Mieth C, Corcoran M, Lembo F, Hallström M, Hartman J, Mazure N, Weide T, Grandér D, Borg J, Uhlén P, Holmgren L
Nature Communications 2014 August;5
Nature Communications 2014 August;5
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Cell lysates at 50 µg per lane
- Sample type
- 293HEK cell transfected with GFP-Rab1a
- Primary Ab dilution
- 1/1,000
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Cells were fixed with 4% PFA
- Sample type
- COS-7
- Primary Ab dilution
- 1/50
- Conjugate
- Unconjugated