Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- PA5-62364 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SAM68 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: YDDTYAEQSY EGYEGYYSQS QGDSEYYDYG HGEVQDSYEA YGQDDWNGTR PSLKAPPARP VKGAYR Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 95%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SAM68 in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a SAM68 Polyclonal Antibody (Product # PA5-62364). Remaining relative intensity is presented.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of MCF7 (Lane 1), MCF7 treated with Insulin (1nM for 24hr) (Lane 2), HeLa (Lane 3), Jurkat (Lane 4), A-431 (Lane 5), U-87 MG (Lane 6) and MOLT-4 (Lane 7). The blot was probed with Anti-SAM68 Polyclonal Antibody (Product # PA5-62364, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 57 kDa band corresponding to SAM68 was detected across the cell lines tested and the expression was also found to increase upon Insulin treatment in MCF7 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SAM68 in human cell line A-431 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a SAM68 Polyclonal Antibody (Product # PA5-62364).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SAM68 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SAM68 Polyclonal Antibody (Product # PA5-62364) at 2µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SAM68 in human endometrium and skeletal muscle tissues using SAM68 Polyclonal Antibody (Product # PA5-62364). Corresponding KHDRBS1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SAM68 in human endometrium using SAM68 Polyclonal Antibody (Product # PA5-62364) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SAM68 in human skeletal muscle using SAM68 Polyclonal Antibody (Product # PA5-62364) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP) assay of endogenous SAM68 protein using Anti-SAM68 Antibody: ChIP was performed using Anti-SAM68 Rabbit Polyclonal Antibody (Product # PA5-62364, 5 µg) on sheared chromatin from HeLa cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to GAPDH, NeuroD1 and cMYC transcriptional start sites, and SAT alpha satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.